Ononin alleviates brain ischemic injury by modulating the Th17/Treg balance
- VernacularTitle:芒柄花苷调控Th17/Treg失衡改善脑缺血损伤
- Author:
Jia-hong GAO
1
;
Li-hua HUANG
1
;
Jin-qian SONG
1
;
Tao PANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Ononin; Th17/Treg immune balance; is-chemic stroke; Foxp3 promoter; tMCAO model; neu-roimmune modulation
- From: Chinese Pharmacological Bulletin 2025;41(10):1835-1842
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the im-munomodulatory effects of Ononin on Th17/Treg bal-ance and inflammatory responses,and to evaluate its neuroinmune regulatory role in ischemic stroke.Methods A Foxp3 promoter-driven luciferase reporter assay was used to assess the effect of Ononin on Foxp3 transcriptional activity.The effect of Ononin on the dif-ferentiation of na?ve CD4+T cells into Th 17 and Treg subsets was evaluated by flow cytometry.A transient middle cerebral artery occlusion(tMCAO)model was established in mice to evaluate the effects of Ononin on infarct size,neurological recovery,body weight resto-ration,and survival rate.Flow cytometry and RT-PCR were conducted to elucidate the immunological mechanisms underlying Ononin's effects.Results On-onin significantly enhanced Foxp3 promoter activity,promoted the differentiation of na?ve CD4+T cells into Treg cells,and suppressed Th17 polarization.In the tMCAO model,Ononin significantly reduced acute in-farct size,improved survival,ameliorated long-term neurological deficits,and increased Treg cell propor-tions in ischemic brain tissue.Conclusions Ononin modulates peripheral and central inflammation by resto-ring the Th17/Treg immune balance,thereby exerting significant immunomodulatory effects.Its therapeutic benefit in ischemic stroke is closely linked to immune balance restoration.
