Effect of Astragalus polysaccharide on the proliferation of rat intestinal mucosal microvascular endothelial cells by regulating VEGF/VEGFR pathway
10.16303/j.cnki.1005-4545.2025.07.12
- VernacularTitle:黄芪多糖调控VEGF/VEGFR通路对大鼠肠黏膜微血管内皮细胞增殖的影响
- Author:
Haotong GUO
1
;
Zihan ZHAO
;
Chang QIAO
;
Mengyu FAN
;
Weichao MA
;
Xiang MU
;
Bo FENG
;
Qian ZHANG
Author Information
1. 北京农学院动物科学技术学院,北京 102206
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharide;
microvascular endothelial cells;
cell proliferation;
VEGFA;
PERK;
rat
- From:
Chinese Journal of Veterinary Science
2025;45(7):1443-1449
- CountryChina
- Language:Chinese
-
Abstract:
This study explored whether Astragalus polysaccharide(APS)can regulate the VEGF/VEGFR signaling pathway to affect the proliferative activity of rat intestinal mucosal microvascu-lar endothelial cells(RIMMVECs).RIMMVECs were isolated from newborn rats,then purified and treated with APS at concentrations of 0.1,1.0,10.0,100.0,1 000.0,and 10 000.0 mg/L.MTT was used to determine the effect of APS on RIMMVECs proliferation and screen for the optimal concentration of APS.Subsequently,flow cytometry was used to detect the changes in cell cycle to evaluate the stage of action of APS on the cell cycle in RIMMVECs.Then,the ELISA was used to detect the changes of VEGFA in cell supernatant to evaluate the potential of cell proliferation and angiogenesis.The changes in fluorescence intensity of Fluo-8AM was observed using fluorescence microscopy to evaluate intracellular Ca2+levels.Finally,Western blot was used to detect the ex-pression of PERK in RIMMVECs to analyze the possible mechanism of APS.The results showed that 100 mg/L APS significantly enhanced the proliferative activity of RIMMVECs,increased the content of VEGFA in the cell supernatant,the intracellular Ca2+levels,and the expression of PERK protein,indicating that APS promotes the proliferation of RIMMVECs,which may be a-chieved by promoting the expression of VEGFA and activating the ERK pathway.
