Establishment of a monoclonal antibody-based competitive chemiluminescent en-zyme-linked immunosorbent assay for detection of Senecavirus A antibodies
10.16303/j.cnki.1005-4545.2025.07.07
- VernacularTitle:基于单抗的A型塞内卡病毒抗体竞争化学发光酶联免疫检测方法的建立
- Author:
Zhenyuan MA
1
;
Ruoqian YAN
1
;
Mao CHAI
1
;
Shujuan WANG
1
;
Xueli ZHAO
1
;
Haibo YANG
1
;
Dongfang WANG
1
;
Ying LIU
1
;
Cui WANG
1
Author Information
1. 河南省动物疫病预防控制中心河南省重大动物疫病监测预警及防控重点实验室,河南郑州 450008
- Publication Type:Journal Article
- Keywords:
Senecavirus type A;
VP2;
VP3;
competitive CLEIA;
quantitative
- From:
Chinese Journal of Veterinary Science
2025;45(7):1402-1410
- CountryChina
- Language:Chinese
-
Abstract:
In order to establish a competitive chemiluminescent enzyme-linked immunoassay for rapid and quantitative detection of Senecavirus A antibodies,the polystyrene plate was coated with inactivated Senecavirus A antigen,and the monoclonal antibodies against Senecavirus A VP2 and VP3 proteins labeled by horseradish peroxidase(HRP)were used as the competitive enzymic anti-bodies of the antibodies in the serum samples.The standard curve of the calibrator prepared by di-lution of positive serum was drawn to achieve quantitative detection.The successfully established SVA competitive CLEIA reported the result within 45 minutes.The maximum dilution of 1∶2 048 for calibrator serum was still detectable with no cross-reaction with the standard positive serum of other five kinds of virus antigens such as foot-and-mouth disease.The coefficient of variation within batches was less than 10％,and the coefficient of variation between batches was less than 15％,which showed good repeatability and stability.The positive and negative coincidence rates were 95.30％and 97.57％,respectively,and the total coincidence rate was 96.88％,showing high consistency.The SVA competitive CLEIA assay established in this study can be used for the rapid quantitative detection of Senecavirus A antibodies,filling the gap in the domestic rapid quantitative detection of SVA antibodies.
