Expression Level and Proliferation-regulating Function of KCNJ14 in Glioma Cells
10.3870/j.issn.1672-0741.24.11.006
- VernacularTitle:KCNJ14在神经胶质瘤细胞中的表达及其对细胞增殖的影响
- Author:
Ruonan GUO
1
;
Min XIANG
;
Xiaobai WU
Author Information
1. 江苏大学附属医院中心实验室,镇江 212001
- Publication Type:Journal Article
- Keywords:
glioma;
KCNJ14;
cell proliferation;
cell cycle
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(5):665-671,693
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine KCNJ14 gene expression in human glioma cells and assess its impact on U251 cell pro-liferation,while exploring the potential mechanism involved.Methods The protein expression levels of KCNJ14 in different hu-man glioma cell lines(U87,U251,SNB19,and LN229)were analyzed by Western blotting.KCNJ14 was knocked down and over-expressed in U251 cells using siRNA and plasmid transfection,respectively.Subsequently,protein expression levels,cell prolif-eration capacity,and the regulation of cell cycle related proteins were measured in each group.The association between KCNJ14 mRNA expression and clinical survival prognosis in glioma patients was evaluated through statistical analysis of public databas-es.Results KCNJ14 protein expression varied across different human glioma cell lines,with the highest level observed in U251 cells.Inhibition of KCNJ14 suppressed U251 cell proliferation and impaired cell cycle progression.Bioinformatics analysis re-vealed that KCNJ14 mRNA expression was significantly associated with clinical characteristics and survival outcomes in glioma patients.Conclusion KCNJ14 exhibits differential expression in glioma cells and is negatively associated with patient prognosis.Mechanistically,it may regulate glioma cell proliferation by modulating cell cycle related proteins.
