Mechanism of LCN2-regulated Microglial Polarization in Intracerebral Hemorrhage Based on Ferroptosis
10.3870/j.issn.1672-0741.24.11.011
- VernacularTitle:基于铁死亡探讨LCN2调控小胶质细胞极化在脑出血中的作用机制
- Author:
Tingting LIN
1
;
Hongmiao XU
1
;
Fujun ZUO
1
Author Information
1. 长沙市第四医院(长沙市中西医结合医院)神经外科(二区),长沙 410200
- Publication Type:Journal Article
- Keywords:
intracerebral hemorrhage;
LCN2;
ferroptosis;
microglia;
cell polarization
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(5):645-649
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of ferroptosis induced by lipocalin2(LCN2)and microglial po-larization in intracerebral hemorrhage(ICH).Methods Forty SD rats were divided into sham operation group,ICH group,LCN2 low expression group and Liproxstatin-1 group,with 10 rats in each group.The morphological structure of the brain was observed and recorded,and the cranial nerve function(NIHSS)score was performed.Immunofluorescence staining was used to detect the expression of microglia marker protein(Iba1),and M1 and M2 microglia marker proteins(CD68 and CD206)in brain tissue.Western blot was used to detect the protein levels of LCN2,ferroptosis marker proteins[solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4)]in brain tissue.The contents of ferrous ion(Fe2+)and malondialdehyde(MDA)in brain tissue were detected by ELISA kits.Results Compared with the sham operation group,the expression of LCN2 protein,the intracerebral hemorrhage,the NIHSS score increased(all P＜0.05),and the expression of CD68 and CD206 in the microglia increased,the expression of ferroptosis-related proteins(SLC7A11 and GPX4)decreased,and the content of Fe2+and MDA increased in the brain tissue of the ICH group(all P＜0.05).Compared with the ICH group,the expression of LCN2 pro-tein,the intracerebral hemorrhage,the NIHSS score and the expression of CD68 in microglia significantly decreased,and the ex-pression of CD206 significantly increased,the expression of SLC7A11 and GPX4 increased,the contents of Fe2+and MDA de-creased in the brain tissue of the LCN2 low expression group(all P＜0.05).The expression of CD68 in brain tissue of Liprox-statin-1 group was significantly decreased,and the expression of CD206 was significantly increased as compared with ICH group(P＜0.05).Conclusion Down-regulation of LCN2 can promote microglial cell polarization by inhibiting ferroptosis,and im-prove nerve function injury in ICH rats.
