Screening and enzyme activity analysis of chitinase-producing strains from tick-de-rived Bacillus
10.16303/j.cnki.1005-4545.2025.07.06
- VernacularTitle:在蜱源性芽孢杆菌中产几丁质酶菌株的筛选及酶活性检测
- Author:
Gejile HU
1
;
Fuli YU
;
Jianzhong LIANG
;
Yuxin LIU
;
Chula KA
;
Lageqi YI
;
Rigele TE
;
Rina SU
;
Fang LIU
;
Riletu GE
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018
- Publication Type:Journal Article
- Keywords:
Bacillus;
chitinase;
enzymatic activity;
Dermacentor nuttalli
- From:
Chinese Journal of Veterinary Science
2025;45(7):1394-1401
- CountryChina
- Language:Chinese
-
Abstract:
The biological activity of chitinase in degrading chitin has garnered extensive attention,particularly for its potential applications in biological control.This study utilized four spore-form-ing Bacillus strains isolated from Dermacentor nuttalli ticks collected in the Hulunbuir region.Traditional bacterial culture methods were employed for isolation and identification,followed by 16S rRNA sequencing and phylogenetic analysis of the purified cultures.chitin-hydrolyzing strains were screened using colloidal chitin plates,and specific chitinase genes were detected via PCR.Fer-mentation was conducted at 37.0 ℃ for 4 d,and the supernatants were subjected to enzyme activity analysis using the DNS method.Four Gram-positive Bacillus strains were successfully isolated from tick tissue samples,they were identified as B.proteolyticus,B.paramycoides,B.thuringien-sis,and B.cereus,and renamed IMH/B-1,IMH/P-1,IMH/T-1,and IMH/C-1,respectively.PCR a-nalysis detected chitinase genes in B.proteolyticus and B.thuringiensis,while B.cereus and B.pa-ramycoides lacked these genes.However,three strains B.proteolyticus,B.thuringiensis,and B.ce-reus demonstrated significant(P＜0.01)chitin degradation activity on colloidal chitin.Enzyme ac-tivity assays revealed that chitinase activity ranged from 1.292 to 2.032 U/mL,with B.proteolytic-us exhibiting the highest activity 2.032 U/mL,followed by B.cereus 1.496 U/mL and B.thuring-iensis 1.324 U/mL.This study provides a foundation for further research and application of chiti-nase-producing Bacillus strains.
