lncRNA NRON induces myocardial fibrosis in mice with myocardial infarction by regulating the TGF-β/Smad signaling pathway
10.12007/j.issn.0258-4646.2025.10.011
- VernacularTitle:lncRNA NRON通过调节TGF-β/Smad信号通路诱导心肌梗死小鼠心肌纤维化
- Author:
Chao YANG
1
;
Tao SU
;
Di JIA
;
Yan LIN
;
Hao CHENG
;
Qi ZHANG
;
Jing LIANG
;
Chunjing ZHANG
Author Information
1. 齐齐哈尔医学院医学技术学院生物化学教研室,黑龙江 齐齐哈尔 161006;齐齐哈尔医药科学研究所博士后科研工作站,黑龙江 齐齐哈尔 161006
- Publication Type:Journal Article
- Keywords:
long non-coding RNA;
NRON;
TGF-β/Smad;
myocardial infarction;
myocardial fibrosis
- From:
Journal of China Medical University
2025;54(10):926-930
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of long non-coding RNA(lncRNA)NRON on myocardial fibrosis in mice with myocardial infarction(MI).Methods Thirty-two C57/BL6 mice were randomly assigned to a Sham group,MI group,MI+shNRON group or MI+NC group,with eight mice in each group.The expression level of lncRNA NRON in myocardial tissue of mice was detected by real-time quantitative PCR.Hematoxylin and eosin staining,Masson's trichrome staining,and immunohistochemistry were used to detect the degree of myocardial injury,myocardial fibrosis,and the expression level of collagen Type Ⅰ(col Ⅰ).Western blotting was used to detect the protein expression levels of TGF-β1,p-Smad2,and p-Smad3 in myocardial tissue of the mice.Results Compared with the Sham group,the expression of NRON,col Ⅰ,TGF-β1,p-Smad2,and p-Smad3 proteins were increased in the MI group.Compared with the MI group,the expression of NRON,the degree of myocardial damage and fibrosis,the expression of col Ⅰ,TGF-β1,p-Smad2,and p-Smad3 proteins were decreased in the MI+shNRON group.Conclusion Down-regulation of lncRNA NRON can alleviate myocardial injury and inhibit myocardial fibrosis in mice with MI,and the molecular mechanism may be related to inhibition of the TGF-β/Smad signaling pathway.
