Effect of Sechang Zhixie Powder on the Intestinal Mucosal Barrier of Acute Diarrhea in Mice
10.3870/j.issn.1004-0781.2025.11.002
- VernacularTitle:涩肠止泻散对急性腹泻小鼠肠黏膜屏障的影响
- Author:
Yuyan WANG
1
;
Chunhui QU
;
Yumeng PENG
;
Wenting HU
;
Lan WANG
;
Yingfei LI
;
Zhiyong LI
Author Information
1. 山东中医药大学中医学院,济南 250355;中国中医科学院中药研究所,北京 100700
- Publication Type:Journal Article
- Keywords:
Sechang Zhixie Powder;
Acute diarrhea;
Mucosal barrier;
Aquaporin-3
- From:
Herald of Medicine
2025;44(11):1736-1744
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of Sechang Zhixie Powder(SCZXS)on mice with acute diarrhea caused by castor oil.Methods The mice were randomly divided into blank control group,model control group,montmorillonite powder group(1.4 g·kg-1),SCZXS-L group(0.9 g·kg-1),SCZXS-M group(1.8 g·kg-1)and SCZXS-H group(3.6 g·kg-1),10 mice in each group.After continuous administration of 7 days,the acute diarrhea model of mice was prepared by oral administration of castor oil(0.01 mL·g-1).The diarrhea of mice was observed within 4 hours of castor oil administration,and the rate of loose stool,degree of loose stool,and diarrhea index were calculated;the levels of DAO,D-LDH,VIP,and SS in the colon were determined by enzyme-linked immunosorbent assay;The morphological changes in the colon tissue of mice were observed after HE staining and the thicknesses of the mucosal and muscular layers of the colon were quantified;AB-PAS staining was performed to observe the effect on mucin in the colon;and the expression of AQP3,Occludin,and ZO-1 in the colon were quantified by immunohistochemistry.Results Compared with the model control group,the rate of loose stool,degree of loose stool,and diarrhea index of the mice in the SCZXS groups tended to decrease,but the difference was not statistically significant.Compared with the model control group,the flat luminal surface of the mice in the SCZXS-M and SCZXS-H group were significantly thickened(P<0.01),and the amount of VIP in the colon was significantly decreased(P<0.05,P<0.01),and that of DAO in the colon was significantly decreased(P<0.01),Occludin and ZO-1 expression were significantly increased(P<0.01),the mucin area ratio was significantly increased(P<0.05)in the SCZXS-M group,and AQP3 expression was significantly increased(P<0.05)in the SCZXS groups.Conclusions SCZXS can improve acute diarrhea induced by castor oil in mice.and its mechanism may be related to the regulation of gastrointestinal hormones and AQP3.In addition,SCZXS improves intestinal damage caused by diarrhea and protects the intestinal barrier.
