miR-372-mediated downregulation of Runx2 inhibits osteoblasts mineralization
10.12007/j.issn.0258-4646.2025.09.002
- VernacularTitle:miR-372下调Runx2表达抑制成骨细胞矿化
- Author:
Jiubin ZHANG
1
;
Yuehong XU
;
Jun YAN
;
Yuduo LIU
Author Information
1. 聊城市人民医院骨科,山东 聊城 252000;北京积水潭医院聊城医院骨科,山东 聊城 252026
- Publication Type:Journal Article
- Keywords:
Runx2;
miR-372;
osteoblast;
mineralization
- From:
Journal of China Medical University
2025;54(9):775-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effect of miR-372 on Runx2 expression and its impact on osteoblast mineralization.Methods Bioinformatic software(Target Scan,miRanda,RNAhybrid)was used to predict microRNAs(miRNAs)that target Runx2,the binding ability of miR-372 to Runx2 was confirmed by dual-luciferase reporter gene experiments.Western blotting analysis was performed to evaluate the regulatory effects of miR-372 on Runx2 protein expression.Osteoblast mineralization was induced using ascorbic acid andβ-glycerophosphate treatment.The functional impact of miR-372 mediated Runx2 regulation on osteoblast mineralization was assessed by measuring Alkaline Phosphatase(ALP)activity and mineralized nodules formation.Results Dual-luciferase reporter gene experiments confirmed direct binding of miR-372 to the Runx2.Western blotting indicated that overexpression of miR-372 in transfected osteoblasts signficantly suppressed Runx2 protein expression,while silencing miR-372 led to elevated Runx2 protein levels.After mineralization induction,ALP activity and mineralization quantitative detection showed miR-372 reuced ALP activity(P＜0.05)and inhibited minerali-zation(P＜0.05)by suppressing Runx2 expression,which was more obvious especially after Runx2 silencing.Conclusion miR-372 can inhibits osteoblast mineralization by downregulating Runx2 expression.
