Value of the Nrf2/Keap1/p62 Pathway in Patients with Postoperative Pulmonary Inflammation after Thoracic Surgery
10.11969/j.issn.1673-548X.2025.09.021
- VernacularTitle:Nrf2/Keap1/p62通路在胸外科术后肺部炎症患者体内的价值
- Author:
Lingling BU
1
;
Liang LI
;
Yuqing DUAN
Author Information
1. 230012 合肥,安徽中医药大学研究生院
- Publication Type:Journal Article
- Keywords:
Nrf2/Keap1/p62 pathway;
Postoperative thoracic surgery;
Pulmonary inflammation;
Prognosis
- From:
Journal of Medical Research
2025;54(9):123-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of molecules related to the Nrf2/Keap1/p62 signaling pathway in patients with postoperative pulmonary inflammation after thoracic surgery,and explore its relationship with prognosis.Methods Sixty patients with postoperative pneumonia after thoracic surgery who met the criteria in the Department of Thoracic Surgery of Taihe County Hospital of Traditional Chinese Medicine from June 2023 to June 2024 were selected as the research subjects.Venous blood was collected from the pa-tients on the day before surgery,the first day after surgery,the third day after surgery,and the seventh day after surgery to detect the lev-els of superoxide dismutase(SOD),nitric oxide(NO),malondialdehyde(MDA),reactive oxygen species(ROS)in peripheral blood,as well as the expression of Nrf2,Keap1,and p62 mRNA.The correlations between these indicators and the prognosis of postoperative pulmonary infection after thoracic surgery were analyzed.Results The SOD level of the patients decreased after the operation compared with that before the operation,reaching the lowest value on the first day after the operation.The levels of MDA and ROS increased after the operation,but slowly decreased on the seventh day after the operation.There was no significant change in the NO level compared with that before the operation.Compared with before the operation,the expression levels of Nrf2 and Keap1 in the patients decreased signifi-cantly on the first day after the operation,and then slowly increased.The expression level of p62 increased to the peak on the first day af-ter the operation,and then decreased.The results of lung function index measurement showed that the patients had pulmonary infection af-ter the operation,and the lung function level decreased significantly compared with that before the operation.Through ROC curve analy-sis,the dynamic changes of SOD,ROS,Nrf2,Keap1 and p62 in the patients with pulmonary inflammation after thoracic surgery have good predictive value for prognosis.Conclusion The changes of Nrf2/Keap1/p62 signaling pathway-related factors in patients with post-thoracic surgery-induced pulmonary inflammation are significantly correlated with the prognosis of these patients.Such changes can effectively predict the prognosis of patients from the perspective of the expression levels of related genes.
