Immunoregulation Mechanism of Lianqin Jiedu Formula based on Network Pharmacology
10.11969/j.issn.1673-548X.2025.09.016
- VernacularTitle:基于网络药理学研究连芩解毒方的免疫调节机制
- Author:
Yuanxiong LONG
1
;
Mi HAN
Author Information
1. 410007 长沙,湖南中医药大学第一附属医院
- Publication Type:Journal Article
- Keywords:
Network pharmacology;
Molecular docking;
Lianqin jiedu formula;
Immunoregulation
- From:
Journal of Medical Research
2025;54(9):91-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunomodulatory mechanism of lianqin jiedu formula based on network pharmacology meth-ods.Methods Using Troditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and GeneCards da-tabases,the active ingredients and potential targets of lianqin jiedu formula was obtained,as well as immune regulation related targets,and the intersection targets was analyzed.The protein-protein interaction(PPI)network and compound target network was constructed,key active ingredients and core action targets were screened.Molecular docking technology was used to verify the binding activity between key active components and core targets,and the intersection targets were analyzed for Gene Ontology(GO)function and Kyoto Encyclope-dia of Genes and Genomes(KEGG)pathway enrichment using the The Database for Annotation,Visualization,and Integrated Discovery(DAVID)database.Results The Lianqin jiedu formula consists of 143 components and 164 targets,of which 130 components and 79 targets were related to immunoregulation,accounting for 90.91％and 48.17％respectively;among them,there were 13 key compounds and 18 core target genes.The top 4 core targets with the highest degree values showed good binding affinity with the top 3 key compounds.The lianqin jiedu formula was involved in immunoregulation and mainly affects 272 biological processes and 58 pathways.Conclusion The lianqin jiedu formula may exert its immunoregulation effects by targeting core targets such as tumor necrosis factor(TNF)and inter-leukin-6(IL-6)through key compounds such as quercetin,kaempferol,and β-sitosterol,affecting biological processes such as pro-tein phosphorylation,cytoplasm,and nucleus,and regulating key pathways such as metabolic pathways.This study provides a reference for the treatment of subsequent immune diseases.
