Expression Level of PDIA3 in Endometrial Cancer and Its Impact on Ishikawa Cells
10.11969/j.issn.1673-548X.2025.09.025
- VernacularTitle:PDIA3在子宫内膜癌中的表达水平及对Ishikawa细胞的影响
- Author:
Mengxuan LI
1
;
Qianyu HOU
;
Cong XIN
Author Information
1. 221000 徐州医科大学附属医院妇科病区
- Publication Type:Journal Article
- Keywords:
Endometrial cancer;
PDIA3;
Ishikawa cells;
TCGA database
- From:
Journal of Medical Research
2025;54(9):147-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression level,clinical significance of protein disulfide isomerase A3(PDIA3)in endome-trial cancer(EC)tissues,and its effects on Ishikawa cells.Methods Using the TCGA database to analyze the differential expression levels of PDIA3 in different endometrial tissues and explore its relationship with clinical pathological parameters and prognosis of EC pa-tients;Using immunohistochemistry(IHC)to detect the expression level of PDIA3 in clinical endometrial tissue,analyze the clinical pathological parameters,prognosis,and risk factors affecting the prognosis of EC patients with different expression levels;Using Transwell assay,scratch assay,and CCK-8 assay to detect the proliferation,invasion,and migration ability of Ishikawa cells with knockdown PDIA3 expression.Results The expression level of PDIA3 in EC tissue was higher than that in normal endometrial tissue in the TCGA database and clinical specimens(P＜0.05).The expression level of PDIA3 in EC tissue in the G3 grade group was higher than that in the G1 and G2 grade groups in the TCGA database(P＜0.05).The expression level of PDIA3 in EC tissue in FIGO stage Ⅱ-Ⅳ group was higher than that in the stage Ⅰ group,the group with lymph node metastasis was higher than that without lymph node metastasis,and the highly differentiated group was higher than the poorly differentiated group in clinical specimens(P＜0.05);COX regression analysis showed that Federation International of Gynecology and Obstetrics(FIGO)staging and PDIA3 expression level were the independent risk factors of prognosis in EC patients;the survival time of EC patients with high expression of PDIA3 in the TCGA database was shorter than that of the low expression group(P＜0.05),and the survival rate of clinical patients with positive PDIA3 was shorter than that of the neg-ative group(P＜0.05);Transwell assay,scratch assay,and CCK-8 assay showed that the invasion and migration ability of cells in the PDIA3 inhibition group was significantly reduced compared to the normal group and negative control group(P＜0.05).Conclusion PDIA3 is highly expressed in EC tissues and is associated with the malignancy of tumors and poor prognosis of patients;targeted inhibition of PDIA3 expression can reduce the proliferation,invasion,and migration ability of Ishikawa cells.
