Bioinformatics-based Analysis of SLC7A5 Expression and Prognostic Value in Breast Cancer
10.11969/j.issn.1673-548X.2025.09.008
- VernacularTitle:基于生物信息学分析SLC7A5在乳腺癌中的表达和预后价值
- Author:
Liu WANG
1
;
Zhijun MA
Author Information
1. 810001 西宁,青海大学研究生院
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Slc7a5;
Bioinformatics;
Prognosis;
Immune checkpoint
- From:
Journal of Medical Research
2025;54(9):36-43,197
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the correlation between SLC7A5 expression and clinicopathological features in breast cancer by bioinformatics,and to provide reference for the treatment and prognosis evaluation of breast cancer.Methods The expression of SLC7A5 in breast cancer was analyzed by bioinformatics in Timer2.0,The cancer genome atlas(TCGA),UALAN in gene expression profiling in-teractive analysis 2(GEPIA2)and human protein atlas(HPA).Human protein atlas(HPA)and other databases to obtain the expression characteristics of SLC7A5 in breast cancer.Western blot was used to detect the expression of SLC7A5gene in normal breast cells MCF-10A and breast cancer cell line MCF-7.the prognostic value of SLC7A5 in breast cancer was analyzed by using R software,and double-validated in Kaplan-Meier plotter and gene expression omnibus(GEO)databases.validation,as well as analyzing the correla-tion between its expression and different pathological features of breast cancer patients.The correlation between SLC7A5 and tumor muta-tion burden(TMB)and immunodetection point-related genes was analyzed using R language,and gene set enrichment analysis(GSEA)was performed,and the results were further visualized.Results The expression of SLC7A5 was significantly elevated in a variety of solid malignant tumors,including breast cancer,and the overall survival of breast cancer patients with high expression of SLC7 A5 was signifi-cantly shortened.The expression level of SLC7A5 in breast cancer was significantly correlated with estrogen receptor(ER),progesterone receptor(PR),lymph node metastasis,and molecular typing of breast cancer.The results showed that the expression of SLC7A5 in breast cancer was positively correlated with TMB(r=0.33,P＜0.001).The results of the immune checkpoint analysis showed that the expres-sion of SLC7 A5 was significantly correlated with the expression of programmed death-1(PD-1),cytotoxic T lymphocyte-associated protein 4(cytotoxic T lymphocyte-associated protein 4(cytotoxic T lymphocyte-associated protein 4),and the expression of SLC7A5 was significantly correlated with the expression of ER,PR,lymph node metastasis and molecular typing.cytotoxic T lymphocyte-associ-ated antigen 4(CTLA4)and B and T lymphocyte attenuator(BTLA).GSEA enrichment analysis showed that SLC7 A5 was mainly associ-ated with the interferon γ response(IFN-γ)related pathway.Conclusion SLC7A5 is up-regulated in breast cancer,and its expres-sion level correlates with poor prognosis,which may affect the progression of breast cancer by participating in the IFN-γ-related path-way to promote the expression of immune checkpoints,and it may be used as a biomarker for prognostic assessment and guidance of treat-ment for breast cancer patients.
