Influence of optimal selection of planktonic apparatus on sperm ultrastructure and IVF-ET outcome analysis: a randomized controlled trail
10.3760/cma.j.cn101441-20200508-00266
- VernacularTitle:浮游器筛选后精子超微结构变化和IVF-ET结局分析：一项随机对照试验
- Author:
Xiaomin CAO
1
;
Li LIU
1
;
Jianyong DI
1
;
Ye LIU
1
;
Meizi ZHANG
1
;
Fengqin XU
1
Author Information
1. 天津市第一中心医院生殖医学科 300192
- Publication Type:Journal Article
- Keywords:
Density gradient method;
Upstream method;
Superstructure sperm ultrastructure;
Fertilization in vitro;
Embryo transfer;
Sperm optimal plankton
- From:
Chinese Journal of Reproduction and Contraception
2021;41(7):610-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find a suitable semen treatment method in in vitro fertilization (IVF). Methods:A prospective randomized double-blind design was performed, 300 infertile couples with female factors and no abnormal fertility test were selected for IVF-embryo transfer (ET), the patients were randomly divided into float group (FY group, n=100), density gradient group (DG group, n=100) and upstream group (SU group, n=100). The remaining sperm after fertilization were collected from three groups, and sections of electron microscope were prepared. The ultrastructure of sperm was observed by transmission electron microscope. The differences among the three groups of selected sperm DNA fragment index (DFI) and fertilization rate, cleavage rate, high-quality embryo rate and pregnancy rate during IVF-ET were compared. Results:The sperm DFI of the three groups after treatment was statistically different ( P=0.02), and the sperm DFI of the FY group was significantly lower than that of DG group and SU group (3.22%±2.73% vs. 8.31%±2.14% vs. 6.43%±2.56%). The rates of sperm head and tail plasma membrane integrity rate in FY group were significantly higher than those in the other two groups (92.0%±24.2% vs. 80.2%±29.5% vs. 73.2%±30.1% and 93.9%±1.2% vs. 80.1%±1.1% vs. 74.9%±1.2%), and the differences were statistically significant ( P=0.01, P=0.03). The high-quality embryo rate and the blastocyst formation rate of FY group were significantly higher than those of DG group and SU group [44.14% (452/1024) vs. 32.30% (292/904) vs. 32.46% (296/912) and 54.40% (396/728) vs. 43.52% (302/694) vs. 46.34% (330/712)], and the differences were statistically significant (all P<0.001). The pregnancy rate and the implantation rate in the fresh cycle FY group were significantly higher than those in DG and SU groups [57.14% (24/42) vs. 33.33% (16/48) vs. 35.56% (16/45) and 50.00% (30/60) vs. 27.45% (14/51) vs. 28.26% (13/46)], and the differences were statistically significant ( P=0.04, P=0.02). The pregnancy rate and implantation rate in the resuscitation cycle FY group were significantly higher than those in the DG and SU groups [52.38% (22/42) vs. 31.25% (10/32) vs. 37.14% (13/35) and 52.38% (22/42) vs. 29.41% (10/34) vs. 30.56% (11/36)], and the difference was statistically significant ( P=0.03, P=0.02). Conclusion:The Plankton method can reduce physical damage to sperm, improve the integrity rate of sperm plasma membrane and decrease sperm DFI, improve assisted reproductive technology outcome.
