Application of Indirect Method in the Establishment Reference Interval for Anti-Miillerian Hormone in Healthy Women of Childbearing Age in Shenzhen Area
10.3969/j.issn.1671-7414.2025.06.036
- VernacularTitle:间接法建立深圳地区育龄期健康女性血清抗缪勒氏管激素参考区间及验证
- Author:
Xiaolin QIN
1
;
Chun DUAN
;
Junxiang LI
;
Yuchi GAO
;
Jie YANG
;
Jianhui TAN
;
Qinglin MA
Author Information
1. 南方医科大学附属深圳市妇幼保健院妇幼研究所,广东 深圳 518028
- Publication Type:Journal Article
- Keywords:
Hoffmann method;
indirect method;
anti-Miillerian hormone;
reference intervals;
Shenzhen area
- From:
Journal of Modern Laboratory Medicine
2025;40(6):196-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and validate the reference interval for serum anti-Müllerian hormone(AMH)in healthy women of childbearing age in Shenzhen using an indirect method based on mathematical statistics.Methods Collected the AMH data for women aged 21～50 in outpatient and physical examination populations in Shenzhen Maternity and Child Health Care Hospital from 2017 to 2023.Grouping by age range of five,first performed normality tested on each group of data,and used the interquartile range method to remove outliers for non-normal data.Then established reference intervals for AMH in different age groups through indirect method(Hoffmann method)and verified them.Compared with the reference interval of the reagent instructions and analyzed the correlation be-tween serum AMH levels and age.Results The correlation coefficient between serum AMH and age in women of childbearing age was-0.642,and the difference was statistically significant(P<0.001).The distribution of AMH levels among women in six age groups was compared,and the difference was statistically significant(H=28 392.655,P<0.05),and AMH levels showed a decreasing trend with age.The reference range of serum AMH at ages 21～25,26～30,31～35,36～40,41～45 and 46～50 yesrs were 0.92～11.30,0.68～9.43,0.38～7.51,0.12～6.93,<4.42 and<1.83ng/ml,respectively,and all reference intervals in each group had been validated.Compared to the age groups provided by the manufacturer,the age groups in this study are more refined,the reference range was narrower,and the interpretation of clinical and laboratory data was more accurate.Conclusion This study used Hoffmann's indirect method for the first time to establish a reference range for AMH in women aged 21～50 years in Shenzhen.The reference interval established is more in line with the actual situation and is a simple and reliable acquisition mode,suitable for promotion and wide application in clinical labo-ratories.
