Study on the Mechanism of Malt Alcoholic Extract in the Treatment of Depression Induced by Chronic Unpredictable Mild Stress in Rats Based on Intestinal Flora
10.3870/j.issn.1004-0781.2025.08.002
- VernacularTitle:基于肠道菌群探究麦芽乙醇提取物对慢性不可预测应激大鼠的抗抑郁作用机制
- Author:
Yindan XIANG
1
;
Ping NI
;
Mengjuan TAO
;
Tianhang LI
;
Yujie ZHOU
;
Huilan XU
;
Bin WANG
;
Qingyuan ZENG
;
Yonggang CHEN
Author Information
1. 湖北中医药大学药学院,武汉 430065;武汉市临床检验中心,武汉 430015
- Publication Type:Journal Article
- Keywords:
Malt;
Intestinal flora;
Depression;
Inflammation;
5-hydroxytryptamine
- From:
Herald of Medicine
2025;44(8):1199-1207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of malt alcohol extract improving depression-like behavior induced by CUMS in rats by regulating gut microbiota.Methods The depression model of rats was established using an 8-weeks CUMS procedure,and the administration group was given low(59.6 mg·kg-1)and high(178.8 mg·kg-1)doses of malt alcohol extract,respectively.The depression-like behavior of rats was evaluated by classic behavioral test.The composition of intestinal microbiota of rats was analyzed by 16S rRNA sequencing.The morphological changes of colon were observed by hematoxylin and eosin(HE),the expression of ZO-1 and Occludin in colon was detected by immunofluorescence(IF),and the expression of IL-10,IL-1βand 5-HT were detected by ELISA.Results The low dose of malt alcohol extract attenuated the depressive behavior and restored the expression of 5-HT in the brain of CUMS rats.16S rRNA sequencing results showed that the diversity and relative abundance of gut microbiota changed after treatment with the low dose of malt alcohol extract.ELISA results showed that the low dose of malt alcohol extract significantly reversed the CUMS-induced reduction of IL-10 and elevation of IL-1 β.HE results showed that the low dose of malt alcohol extract significantly ameliorated CUMS-induced structural damage in colon.IF results showed increased protain expression of intestinal epithelial barrier tight junction proteins ZO-1 and Occludin by the low dose of malt alcohol extract.Conclusion The low dose of malt alcohol extract can ameliorate CUMS-induced depressive-like behavior in rats by modulating intestinal flora,restoring 5-HT expression in the brain,inhibiting inflammation,and repairing the intestinal barrier.
