Experimental Studies on the Bacteriostatic and Anti-Biofilm Effects of Tannic Acid Combination with Imipenem on Carbapenem-Resistant Acinetobacter Baumannii
10.3969/j.issn.1671-7414.2025.06.031
- VernacularTitle:单宁酸与亚胺培南联合对耐碳青霉烯鲍曼不动杆菌的抑菌及抗生物膜作用的实验研究
- Author:
Long OUYANG
1
;
Yaxiong XIAO
Author Information
1. 宜宾市中医医院检验科,四川 宜宾 644600
- Publication Type:Journal Article
- Keywords:
tannic acid;
imipenem;
carbapenem-resistant Acinetobacter baumannii;
minimal inhibitory concentration;
biofilm
- From:
Journal of Modern Laboratory Medicine
2025;40(6):171-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro bacterial inhibitory activity and biofilm effect of the combination of tannic acid and imipenem on carbapenem-resistant Acinetobacter baumannii(CRAB),and provide a theoretical basis for the treatment of CRAB infections with tannic acid in combination with imipenem.Methods Collected 30 non-duplicated CRAB strains from clinical isolates of the First People's Hospital of Yibin from May to December 2022.The minimal inhibitory concentration(MIC)of tannic acid and imipenem on CRAB was detected separately by micro broth dilution method,and the effect of drug combina-tion was evaluated according to the fractional inhibitory concentration index(FICI).The effect of sub-inhibitory concentration of drugs on biofilm formation was observed by semi-quantitative adhesion assay.Changes in the microscopic morphology of mature biofilms under the effect of drugs were observed by scanning electron microscopy.Changes in the expression of biofilm-asso-ciated genes BaP,BfS,OmpA were detected by reverse transscription PCR(RT-PCR)after the administration of tannic acid and imipenem alone and in combination.Results The MICs of tannic acid and imipenem against 30 CRAB strains ranged from 32 to 256 μg/ml,and the FICI values of tannic acid combined with imipenem ranged from 0.625 to 1,which showed additive effects.Compared with the control group,tannic acid combined with imipenem could effectively inhibit the formation of biofilm and destroy the structure of mature biofilm,and the biofilm-related genes of BaP,BfS,OmpA and were expressed at a low level in the strains,and the differences were statistically significant(t=26.32,79.17,29.22,all P<0.05).Conclusion For CRAB,tannic acid in combination with imipenem showed additive effects.The anti-biofilm effect of tannic acid in combination with imipenem may be realized by inhibiting the expression of biofilm-related genes BaP,BfS,OmpA.
