Evaluation of 6 Drug Susceptibility Testing Methods in Detecting the Sensitivity of Carbapenem-resistant Klebsiella Pneumoniae to Tigecycline
10.3969/j.issn.1671-7414.2025.06.029
- VernacularTitle:6种药敏试验方法检测耐碳青霉烯肺炎克雷伯菌对替加环素敏感度的评价研究
- Author:
Fuwei WU
1
;
Wenming HUA
1
;
Tianqi QI
1
;
Jingxian YANG
1
Author Information
1. 航天中心医院检验科,北京 100049
- Publication Type:Journal Article
- Keywords:
carbapenem-resistant Klebsiella pneumonia;
tigecycline;
drug susceptibility testing
- From:
Journal of Modern Laboratory Medicine
2025;40(6):159-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the accuracy of 6 commercial antibiotic susceptibility testing methods in detecting the sensitivity of carbapenems-resistant Klebsiella pneumoniae(CRKP)to tigecycline,and to provide an evidence for clinical laboratory to select the appropriate tigecyline susceptibility tests.Methods Non-duplicate CRKP clinically isolated from Aerospace Center Hospital from June 2023 to February 2024 were collected.60 cases of tigecycline intermediated CRKP[minimal inhibitery concentration(MIC)=4μg/ml]and 60 cases of drug-resistant CRKP(MIC≥8μg/ml)were screened by Vitek-2.The sensitivity of CRKP to tigecycline was detected by different methods.With the MIC test strip(MTS)as the reference method,the methodological evaluation of Vitek-2,commercial broth dilution method,E-test,Kirby-Bauer disk diffusion(KB)and disk combined with resensitization bufferwas carried out under the interpretation standards of the U S Food and Drug Administration(FDA)and the European Commission for Drug Susceptibility Testing(EUCAST),respectively.Results For the intermediate strains,under the FDA criteria,both the commercial broth dilution method and the disk combinedwith resensitization buffer method showed high consistency with the MTS method and were acceptable,whereas under the EUCAST criteria,all five methods were unacceptable.For the resistant strains,no matter what standard was,CA of all five methods were less than 90%,which were unacceptable.For all strains as a whole,the results were consistent with those of the resistant ones.Conclusion Under the current sample size,none of the five methods met the acceptable standards,especially not suitable for the recheck of strains with higher MICs.Commercial broth dilution method have the highest consistency with MTS,and it is worth expanding the sample size for further study.
