The incidence of chromosomal abnormalities in the villus tissue of women with missed abortion by assisted reproductive technology
10.3760/cma.j.cn101441-20200922-00524
- VernacularTitle:辅助生殖技术助孕女性稽留流产绒毛组织各型染色体异常发生率的研究
- Author:
Mingkun MU
1
;
Simin SUN
1
;
Wei ZHENG
1
;
Chen YANG
1
;
Shuheng YANG
1
;
Ruowen ZU
1
;
Linlin ZHANG
1
;
Jinshuang GAO
1
;
Jing LI
1
;
Xingling WANG
1
;
Yichun GUAN
1
Author Information
1. 郑州大学第三附属医院生殖中心 454000
- Publication Type:Journal Article
- Keywords:
Reproductive technology,assisted;
Missed abortion;
Villus chromosome;
Aneuploidy;
Copy number variations
- From:
Chinese Journal of Reproduction and Contraception
2021;41(6):538-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the incidence and classification of chromosomal abnormalities in villi of missed abortion patients with assisted reproductive technology (ART) and natural conception (NC).Methods:Totally 637 patients with missed abortion villi from the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University during January 2016 and January 2020 were collected and divided into ART group and NC group according to the mode of pregnancy in this retrospective cohort study. The ART group was further divided into artificial insemination by husband (AIH), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Next generation sequencing (NGS) was used to detect the copy number variations (CNVs) and chromosome number abnormalities of chorionic villi of missed abortion. Results:Among 637 missed abortion chorionic villi, 45.2% (288/637) of the samples had normal chromosome and 54.8% (349/637) had abnormal chromosome. CNVs accounted for 3.8% (14/637) of the total samples, and chromosome number abnormalities accounted for 52.5% (335/637) of the total samples. The abnormal rates of villi chromosome in ART group and NC group were 59.2% (226/382) and 51.0% (130/255), respectively, and there was no significant difference between ART group and NC group ( P>0.05). The abnormal rates of villus chromosome in AIH group, IVF group and ICSI group were 52.1% (25/48), 58.9% (146/248) and 64.0% (55/86), respectively. Compared with NC group, the abnormal rate of villus chromosome in IVF group and ICSI group was increased, but there was no significant difference ( P>0.008). Conclusion:In general, ART did not increase the incidence of chromosomal abnormalities in missed abortion villi. However, compared with natural pregnancy and AIH assisted pregnancy, IVF/ICSI had a higher chromosomal abnormality in missed abortion villi.
