Establishment and Validation of a Prognostic Model for Hepatocellular Carcinoma Based on Macrophage-Ferroptosis-Related Genes
10.13241/j.cnki.pmb.2025.16.003
- VernacularTitle:基于巨噬细胞-铁死亡相关基因的肝细胞癌预后模型构建
- Author:
Yu-yao WANG
1
;
Yuan-ping LIU
;
Rui-xuan WANG
;
Xuan-he CHANG
;
Han-qing FEI
;
Huan WANG
Author Information
1. 青岛大学基础医学院 山东青岛 266071;军事科学院军事医学研究院生命组学研究所 北京 102206
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Macrophages;
Ferroptosis;
Prognosis;
Tumor microenvironment
- From:
Progress in Modern Biomedicine
2025;25(16):2585-2597
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to integrate RNA-seq data to identify key genes associated with macrophage-ferroptosis and develop a prognostic model for hepatocellular carcinoma(HCC).Methods:We retrieved a dataset from the GEO database containing transcriptome data and clinical information for 163 samples.Macrophage-related genes were identified using WGCNA and immune infiltration analyses.Ferroptosis-related genes from the FerrDbV2 database were intersected with differentially expressed genes to obtain significant macrophage-ferroptosis-related genes.Hub genes were screened via protein interaction network construction,and key genes were identified using four machine learning algorithms.These genes exhibited significant expression differences between cancer and normal tissues and were closely linked to patient prognosis.ROC curve and KM survival analyses were performed,and expression levels were validated at the transcriptome and proteome levels.Results:Four key genes RRM2,KIF20A,PCK2,and PDK4 were identified and evaluated.RRM2 and KIF20A demonstrated high importance in classification prediction models and reliable performance in ROC and KM analyses(AUC＞0.9,P＜0.05).These genes regulate cancer cell proliferation/survival and macrophage polarization/function,influencing the tumor microenvironment and HCC progression.Conclusion:RRM2 and KIF20A regulate cancer cell proliferation and survival,modulate macrophage polarization and function,and influence the immune response in the tumor microenvironment.They can serve as prognostic biomarkers and potential immunotherapy targets for hepatocellular carcinoma.
