PTEN affects embryo implantation by regulating the polarity of endometrial luminal epithelial cells
10.3760/cma.j.cn101441-20200604-00329
- VernacularTitle:蛋白PTEN通过调节子宫内膜腔上皮细胞极性影响胚胎着床
- Author:
Jiali PENG
1
;
Xiaoling LI
1
;
Zhuoni XIAO
1
Author Information
1. 武汉大学人民医院生殖医学中心，湖北省辅助生殖与胚胎发育医学临床研究中心 430060
- Publication Type:Journal Article
- Keywords:
PTEN;
Polarity;
Tight junction;
Endometrial luminal epithelial cells
- From:
Chinese Journal of Reproduction and Contraception
2021;41(4):333-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulation of phosphoatase and tensin homolog deleted on chromosomes 10 (PTEN) in the polarity of endometrial lumen epithelial cells and their effects on embryo implantation.Methods:The differences in PTEN expression and localization between non-receptive endometrial epithelial cells (HEC-1A) and receptive endometrial epithelial cells (RL95-2) were compared by qRT-PCR, Western blotting and immunofluorescence. After the transfection of PTEN siRNA into HEC-1A cells, tight junction (TJ) related proteins, TJ structure, cell motility and adhesive capacity with choriocarcinoma cells (JAR) were detected respectively by Western blotting, transmission electron microscope (TEM), Transwell assay and adhesion assay. After dimethylsulfoxide (DMSO), 17β-estradiol, progesterone, and 17β-estradiol+progesterone were respectively added into HEC-1A in vitro, the PTEN protein expression were detected by Western blotting to study the effect of ovarian hormone on PTEN. Results:Compared with HEC-1A cells, the gene and protein expression levels of PTEN in RL95-2 cells were significantly reduced (both P=0.003), PTEN was mainly located in the nucleus of RL95-2 and cytoplasm of HEC-1A. Compared with the plasmid vector control group, the expression level of TJ related proteins (ZO-1, Occludin, Claudin-4) in HEC-1A cells was significantly reduced ( P<0.001, P=0.038, P<0.001), the length of TJ between cells were reduced ( P=0.046), the ability of migration and invasion were enhanced (both P<0.001), and the adhesion rate to JAR cells was enhanced after knockdown of PTEN in HEC-1A ( P=0.016). Compared with the DMSO blank group, the expression level of PTEN protein in 17β-estradiol group, progesterone group and 17β-estradiol+progesterone group were significantly reduced (all P<0.001), the expression level of PTEN protein in 17β-estradiol+progesterone group was significantly lower than that in both 17β-estradiol group and progesterone group (both P=0.001), and there was no difference in the expression level of PTEN protein between progesterone group and 17β-estradiol group. Conclusion:There are differences in the expression of PTEN in endometrial cells with different receptivity states. 17β-estradiol and progesterone may regulate the TJ structure and cell polarity of endometrial epithelial cells by inhibiting the expression of PTEN in endometrial luminal epithelium, thereby enhancing the endometrial receptivity.
