Antibacterial Effect of Barium Copper Titanate Piezoelectric Material Coupled with Ultrasound in vitro
10.16156/j.1004-7220.2025.05.026
- VernacularTitle:基于超声耦合的钛酸铜钡压电材料体外抗菌效应
- Author:
Hongyu CHEN
1
;
Yi WANG
;
Yushun TAO
;
Biaohong HUANG
;
Weijin HU
;
Shujun LI
;
Qiang WU
;
Yilai JIAO
;
Liao WANG
Author Information
1. 上海交通大学医学院附属第九人民医院骨科,上海市骨科内植物重点实验室,上海 200021
- Publication Type:Journal Article
- Keywords:
implant-associated infection;
piezoelectric materials;
copper;
sonodynamic therapy;
antibacterial effect;
reactive oxygen species
- From:
Journal of Medical Biomechanics
2025;40(5):1281-1287
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of copper-doped barium titanate(BaCuTiO4)piezoelectric materials combined with low-intensity pulsed ultrasound(LIPUS)to activate their piezoelectric-catalytic synergistic effect for treating implant-associated infections.Methods BaCuTiO4 coatings were synthesized on the surface of Ti-6Al-4V substrates using a hydrothermal method,and their surface morphology was characterized by scanning electron microscopy.The piezoelectric characteristics of the coatings were analyzed using a piezoresponse force microscope.An in vitro biofilm model of methicillin-resistant staphylococcus aureus(MRSA)was used,with barium titanate(BaTiO3)coatings serving as the control group.Under LIPUS intervention(1.0 W/cm2,1 MHz,10 min),the bacterial viability was assessed using colony counting to evaluate the antibacterial performance of the BaCuTiO4 coatings.Confocal microscopy was used to observe biofilm viability in different groups,assessing the biofilm removal capability of the coatings.Reactive oxygen species(ROS)generation in each group was detected using Rhodamine b as a probe to evaluate the catalytic efficiency of the coatings in generating ROS.Results Copper doping significantly reduced the piezoelectric coefficient of the coating(from 17.7 pm/V to 7.8 pm/V),bringing its piezoelectric performance closer to the requirements of natural bone tissues.Under LIPUS activation,the BaCuTiO4 coatings increased the generation efficiency of reactive oxygen species by 67.5％and effectively disrupted and removed biofilms formed by MRSA,achieving an antibacterial rate of 90.5％.Conclusions The BaCuTiO4 coatings achieve efficient antibacterial and biofilm-clearing functions through a piezoelectric-catalytic synergistic mechanism.Their piezoelectric properties are well-matched with natural bone tissues,promoting implant osseointegration.
