Research progress of proline as cryoprotectant
10.3760/cma.j.cn101441-20200626-00359
- VernacularTitle:脯氨酸作为细胞冷冻保护剂的研究进展
- Author:
Qingyuan QIN
1
;
Tao LIU
1
;
Jiangxue QU
1
;
Dongming LIU
1
;
Xiaowei ZHANG
1
;
Jie YAN
1
Author Information
1. 北京大学第三医院妇产科生殖医学中心，北京 100191
- Publication Type:Journal Article
- Keywords:
Proline;
Cryoprotectant;
Oocyte
- From:
Chinese Journal of Reproduction and Contraception
2022;42(2):213-217
- CountryChina
- Language:Chinese
-
Abstract:
Though cells are easily damaged and performed metabolic disorders in freezing-thawing process, cryoprotectant can relief the injure, which is widely used in the field of cell frozen biology. As one of osmotic cryoprotectants, in freezing process, proline can penetrate through cell membrane quickly, bind intracellular water hydrogen bonds, inhibit formation of ice crystals at low temperatures, protect structure and function of proteins and cell membranes. It can increase the survival rate in plant cells, animal cells and human somatic cells post freezing-thawing. In germ cell cryopreservation, studies have shown that proline can be used for oocyte cryopreservation, which can improve the survival rate of mouse oocytes after cryopreservation and protect mitochondrial function. However, the reports about the cryopreservation efficiency of proline on sperm are not consistent. By improving the efficiency of cryopreservation in various kinds of cells, proline may be more widely used in the area of cell freezing biology in the future. This paper summarizes the research progress of proline as a natural small molecule cryoprotectant, hoping to help people better understand the cryobiological mechanism of germ cell cryopreservation, and provide a reference for improving fertility cryopreservation technology.
