Role of mitochondrial calcium uniporter in oxidative damage in HEI-OC1 cells
10.3969/j.issn.1006-7299.2025.05.010
- VernacularTitle:线粒体钙单向转运蛋白在HEI-OC1细胞氧化损伤中的作用
- Author:
Zhang ZHAO
1
;
Zhuxiang CHEN
1
Author Information
1. 江汉大学附属湖北省第三人民医院耳鼻咽喉头颈外科(武汉 430033)
- Publication Type:Journal Article
- Keywords:
Mitochondrial calcium uniporter;
HEI-OC1 cells;
Oxidative stress;
Mitochondrial dynamics
- From:
Journal of Audiology and Speech Pathology
2025;33(5):460-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of mitochondrial calcium uniporter(MCU)in oxidative stress injury in HEI-OC1 cells.Methods H2O2 was used to establish the HEI-OC1 oxidative stress damage model,which were divided into the normal control group,H2 O2 group and H2 O2+Ru360(MCU inhibitor)group.The cell viabil-ity of each group was detected by CCK-8 reagent.State of oxidative stress was detected by flow cytometry.Mito-chondrial membrane potential(MMP)and mitochondrial Ca2+levels were observed by applying JC-1 probe and Rhod-2/AM probe,respectively.The protein expression levels of MCU,Drp1,MFN1 and MFN2 were detected by Western blot.Results HEI-OC1 cells of H2 O2 group showed a significant decrease in cell viability with oxidative stress injury.Cellular ROS accumulation,MMP loss accompanied by mitochondrial calcium overload,elevated MCU protein expression,Drp1 mitochondrial translocation and reduced MFN1 and MFN2 expression.In the cells of H2O2+Ru360 group,the high expression of MCU was inhibited,cell viability was increased and ROS levels were reduced.Besides,the mitochondrial Ca2+levels were reduced and mitochondrial homeostasis was restored.Com-pared with the H2O2 group,the differences were all statistically significant(P＜0.05).Conclusion MCU is in-volved in oxidative stress injury in HEI-OC1 cells,and inhibition of MCU may alleviate cellular stress by improving mitochondrial dynamics-related homeostasis.
