Effect of delivery way on reproductive tract microbiota
10.3760/cma.j.cn101441-20201015-00564
- VernacularTitle:分娩方式对生殖道微生物组影响的机理学研究
- Author:
Xing YANG
1
;
Bolun ZHANG
1
;
Manchao LI
1
;
Zhi ZENG
1
;
Xiaoyan LIANG
1
;
Linzhi GAO
1
;
Rui HUANG
1
Author Information
1. 中山大学附属第六医院生殖医学研究中心，广州 510063
- Publication Type:Journal Article
- Keywords:
Female reproductive tract microbiota;
Infertility;
High-throughput nucleotide sequencing;
Cesarean section uterus
- From:
Chinese Journal of Reproduction and Contraception
2021;41(11):1002-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the correlation between the microbial flora differences of the external cervix and endometrium and female infertility.Methods:It was a case-control study involving 14 patients who had delivered previously and underwent assisted reproduction treatment. Patients were divided into cesarean group (9 patients) and vaginal birth group (5 patients) according to their previous delivery method,and the patients' cervix and endometrium were analyzed separately. The total DNA of the two groups origin from external cervical and endometrial tissues were extracted for 16S rRNA analysis, and the sequencing results were further analyzed by bioinformatics and statistics.Results:External cervical and endometrial tissues from each group were sequenced by high-throughput sequencing methods. Twelve kinds of microbial flora at the level of Phylum were detected. Firmicutes had the highest content, and its level in each group was as follows: vaginal birth cervix group > vaginal birth endometrium group> cesarean cervix group > cesarean endometrium group. The abundance of Bacteroidetes was higher in vaginal birth group, while the Proteobacteria was higher in cesarean group. Fourteen dominant bacterial groups were found at the level of family. Lactobacillus had the highest content and its level among four groups was as follows: vaginal birth cervix group > vaginal birth endometrium group > cesarean cervix group > cesarean endometrium group. Further analysis found 13 dominant bacteria genera, Lactobacillus had the highest content, the level among the four groups was as follows: vaginal birth cervix group > vaginal birth endometrium group > cesarean cervix group > cesarean endometrium group. Conclusion:There were differences in the composition and abundance of female reproductive tract microbiota of women after cesarean delivery and vaginal birth. The abundance of genital tract lactobacillus flora of women after cesarean section was significantly lower than that of women after vaginal birth. This study revealed that the difference in the abundance of Lactobacillus and other communities is related to the decreased fecundity of women who had underwent cesarean section. Exploring the methods to balance microbial community environment in the uterus could promote fecundity and assisted reproductive technology success rate in cesarean scar patients.
