Study on the Expression and Clinical Diagnostic Value of Serum lncRNA PANDAR,PICP Levels in Patients with Type 2 Diabetes Nephropathy
10.3969/j.issn.1671-7414.2025.05.011
- VernacularTitle:2型糖尿病肾病患者血清lncRNA PANDAR,PICP水平表达及临床诊断价值研究
- Author:
Yuanzhen CHU
1
;
Yumei ZHANG
1
;
Chenjun ZHENG
1
Author Information
1. 成都医学院第三附属医院/成都市郫都区人民医院内分泌科,成都 611730
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
long non-coding RNA PANDAR;
procollagen type I C-terminal propeptide
- From:
Journal of Modern Laboratory Medicine
2025;40(5):57-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical diagnosis significance of serum long non-coding RNA PANDAR(lncRNA PANDAR)and procollagen type I C-terminal propeptide(PICP)in diabetic nephropathy(DN).Methods A total of 310 patients with type 2 diabetes mellitus(T2DM)admitted to the Third Affiliated Hospital of Chengdu Medical College from January 2022 to December 2023 were selected as the case group,and further divided into DM group(n=190)and DN group(n=120)based on the presence or absence of concomitant kidney disease.60 healthy subjects were selected as the control group.The expression level of lncRNA PANDAR was determined by real time fluorescence quantitative PCR,and the serum PICP level was determined by enzyme-linked immunosorbent assay(ELISA).The expression of lncRNA PANDAR and serum PICP in each group was compared.Logistic regression was used to analyze the influencing factors of DN.ROC curve was used to evaluate the diagnostic value of serum lncRNA PANDAR,PICP and their combination in DN.Results The levels of serum lncRNA PANDAR(4.56±1.26),PICP(147.72±19.20 μg/L)in the case group were higher than those in the control group(2.27±0.61,117.24±12.72 μg/L),and the differences were statistically significant(t=13.758,11.799,all P<0.05).With the increase of chronic kidney disease(CKD)stage,the levels of serum lncRNA PANDAR and PICP gradually increased,and the differences were statistically significant(F=16.790,286.87,all P<0.05).The levels of serum lncRNA PANDA(5.27±1.26)and PICP(159.59±17.10 μg/L)in DN group were higher than those in DM group(4.11±1.03,140.22±16.56 μg/L),the differences were statistically significant(t=-8.847,-9.905,all P<0.05).Multivariate Logistic regression analysis showed that lncRNA PANDA and PICP were independent risk factors for DN in T2DM patients(Wald χ2=11.592,7.880,all P<0.05).The areas under curve(AUC)of serum lncRNA PANDA and PICP combined detection of DN in patients with T2 DM was higher than that of single index detection,and the differences were statistically significant(Z=-2.006,-3.069,all P<0.05).Conclusion The levels of serum lncRNA PANDAR and PICP in patients with DN are increased,and their combined detection can improve the diagnostic efficiency of DN.
