Experimental study on low volume sperm cryopreservation using Strawtop micro-carrier
10.3760/cma.j.cn101441-20191014-00458
- VernacularTitle:自制冷冻片微型载体（Strawtop）微量精液冻存效果的实验研究
- Author:
Rongxiang WANG
1
;
Qiao YU
;
Qiuping PENG
;
Shaofeng CAO
;
Caixia ZHANG
;
Yu LIU
;
Yiwen ZHOU
;
Songguo XUE
Author Information
1. 同济大学附属东方医院生殖医学中心，上海200123
- Publication Type:Journal Article
- Keywords:
Azoospermia;
Severe oligospermia;
Low volume sperm cryopreservation;
Straw method;
Strawtop method
- From:
Chinese Journal of Reproduction and Contraception
2020;40(12):1006-1012
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the cryopreservation effect of the home-made Strawtop micro-carrier method and Straw method for cryopreservation of human low volume sperm.Methods:A prospective study was performed to analyze the post-thaw results of cryopreserved human sperm using the home-made Strawtop method and Straw method. Compare the survival rate, sperm DNA fragmentation index (DFI), ultrastructure, fertilization ability and embryo developmental competence of donated semen samples from the patients who underwent intracytoplasmic sperm injection (ICSI) treatment in Center for Reproductive Medicine, Shanghai East Hospital, Tongji University School of Medicine during the period from May 1, 2018 to June 30, 2018.Results:The survival rate of Strawtop group (46.8%±17.1%) was significantly higher than that of Straw group (23.1%±13.7%, P=0.001). Compared with fresh sperm (18.9%±11.6%), Straw method (33.5%±15.0%) significantly increased the sperm DFI ( P=0.019), while Strawtop method didn't (23.4%±11.7%, P=0.375). The sperm ultrastructure of Strawtop group was superior to that of Straw group. There was no difference among Strawtop, Straw and fresh group in normal fertilization rates, cleavage rates and day 2 high-quality embryo rates. Conclusion:For human low volume sperm cryopreservation, the Strawtop method was simple and convenient. The survival rate of Strawtop method was higher than that of straw method and the sperm could be used for ICSI directly after thawing. The strawtop method is worth to be widely used in clinical practice.
