Effect of salvianolic acid B on high glucose induced necrotic apoptosis of retinal pigment epithelial cells by regulating the receptor-interacting protein kinase 1/receptor-interacting protein kinase 3/mixed lineage kinase domain like protein signaling pathway
10.3969/j.issn.1006-6187.2025.10.008
- VernacularTitle:丹酚酸B调节受体相互作用蛋白激酶1/受体相互作用蛋白激酶3/混合谱系激酶结构域样蛋白信号通路对高糖诱导的视网膜色素上皮细胞坏死性凋亡影响的研究
- Author:
Fei LIU
1
;
Min XUE
;
Yong WANG
Author Information
1. 230601 合肥,安徽第二医学院医学技术学院
- Publication Type:Journal Article
- Keywords:
Salvianolic acid B;
Receptor-interacting protein kinase 1;
Receptor-interacting protein kinase 3;
Mixed lineage kinase domain like protein;
High glucose;
Retinal pigment epithelial cells;
Necrotic apoptosis
- From:
Chinese Journal of Diabetes
2025;33(10):760-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of salvianolic acid B(Sal B)on high glucose(HG)induced necrotic apoptosis of retinal pigment epithelial cells(RPE)by regulating the receptor-interacting protein kinase 1(RIP1)/RIP3/mixed lineage kinase domain like protein(MLKL)signaling pathway.Methods RPE cells ARPE-19 were used as the research object and separated into control(Con)group,HG group,L-Sal B group,M-Sal B group,H-Sal B group,pcDNA3.1-NC group,and pcDNA3.1-RIP1 group.The qRT-PCR method was applied to detect the expression of RIP1,RIP3,and MLKL in ARPE-19 cells in each group.CCK8 was applied to detect ARPE-19 cell survival rate.Flow cytometry was applied to detect cell apoptosis.DCFH-DA fluorescent probe was applied to detect reactive oxygen species(ROS)level.ELISA was applied to detect the expression of tumor necrosis factor-α(TNF-α),interleukin(IL-1β),and IL-6.Western blot was applied to detect the expression of RIP1,RIP3,and MLKL proteins.Results The cell survival rate was lower in the HG group than in the Con group(P<0.05).The cell apoptosis rate,the proportion of ROS-positive cells,TNF-α,IL-1β,IL-6,and the mRNA and protein expressions of RIP1,RIP3,and MLKL were higher in the HG group than in the Con group(P<0.05).The cell survival rates were higher,while the cell apoptosis rates,the proportion of ROS-positive cells,TNF-α,IL-1β,IL-6,and the mRNA and protein expressions of RIP1,RIP3,and MLKL were lower in the L-Sal B,M-Sal B,and H-Sal B groups than in the HG group(P<0.05).The cell survival rates increased successively(P<0.05),while the cell apoptosis rates,the proportion of ROS-positive cells,TNF-α,IL-1β,IL-6,and the mRNA and protein expressions of RIP1,RIP3,and MLKL decreased successively in the L-Sal B,M-Sal B,and H-Sal B groups(P<0.05).The cell survival rate was lower(P<0.05),while the cell apoptosis rate,the proportion of ROS-positive cells,TNF-α,IL-1β,IL-6,and the mRNA and protein expressions of RIP1,RIP3,and MLKL were higher in the pcDNA3.1-RIP1 group than in the H-Sal B and pcDNA3.1-NC groups(P<0.05).Conclusions Sal B may inhibit the necrotic apoptosis of RPE cells induced by HG by suppressing the RIP1/RIP3/MLKL signaling pathway.
