Dehydrocostus lactone ameliorates renal interstitial fibrosis in rats with unilateral ureteral obstruction by inhibiting the TGF-β1/Smad2/3 pathway
10.12464/j.issn.1674-7445.2025171
- VernacularTitle:去氢木香烃内酯通过抑制TGF-β1/Smad2/3通路改善单侧输尿管梗阻大鼠肾间质纤维化
- Author:
Zhongda LI
1
;
Shuhong WANG
;
Hongwei TAN
;
Xiao WANG
;
Jinyang ZHUANG
;
Sheng SHEN
;
Qipeng SUN
Author Information
1. 510317 广州,暨南大学附属广东省第二人民医院器官移植科
- Publication Type:Journal Article
- Keywords:
Dehydrocostus lactone;
Transforming growth factor-β1;
Collagen fiber;
Unilateral ureteral obstruction;
Renal interstitial fibrosis;
Collagen;
α-smooth muscle actin;
Chronic kidney disease
- From:
Organ Transplantation
2025;16(5):763-770
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the action mechanism of dehydrocostus lactone(DHL)on renal interstitial fibrosis(RIF)in rats with unilateral ureteral obstruction(UUO).Methods Forty-four male SD rats were randomly divided into four groups:the sham surgery group(Sham group),the pure drug intervention group(Sham+DHL group),the experimental group(UUO+Vehicle group)and the DHL treatment group(UUO+DHL group),with 11 rats in each group.The rats underwent sham surgery,sham surgery+DHL[10 mg/(kg·d)],UUO modeling+the same volume of solvent and UUO modeling+DHL[10 mg/(kg·d)],respectively.After surgery,DHL or the same volume of solvent was administered by gavage for 14 days starting from day 2 post-surgery.Hematoxylin-eosin(HE)and Masson staining were used to observe the pathological changes in renal tissue.Immunohistochemical staining was performed to detect the expression levels of collagen I,collagen III and α-smooth muscle actin(α-SMA).Western blotting was used to detect the protein levels of the transforming growth factor(TGF)-β1/Smad2/3 pathway.Results Compared with the UUO+Vehicle group,DHL treatment alleviated renal interstitial pathological damage,reduced collagen fiber deposition,and decreased the expression of collagen I,collagen III and α-SMA.It also inhibited the expression of TGF-β1 and Smad2/3 proteins.Conclusions DHL mitigates RIF in rats by inhibiting the TGF-β1/Smad2/3 pathway,providing a new strategy for the treatment of chronic kidney disease.
