A comparative study on the effects of CRH neuron-related brain regions on the behavior of stressed mice
10.16557/j.cnki.1000-7547.2025.05.004
- VernacularTitle:CRH神经元相关脑区对应激小鼠行为影响的比较研究
- Author:
Jinyi XIE
1
;
Junchen LIU
;
Junyu LIU
;
Yuechen YAN
;
Shengxi WU
;
Baolin GUO
Author Information
1. 空军军医大学基础医学院学员五大队,西安 710032
- Publication Type:Journal Article
- Keywords:
periaqueductal gray(PAG);
medial preoptic area(MPA);
corticotropin releasing hormone(CRH);
acute stress;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(5):573-580
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the differential regulatory effects of corticotropin releasing hormone(CRH)neu-rons in periaqueductal gray(PAG)and medial preoptic area(MPA)downstream of paraventricular nucleus(PVN)in mediating stress-related abnormal behaviors.Methods:The anterograde labeled virus AAV2/9-hSyn-DIO-hChR2-EYFP and AAV2/9-mCrh-SV40 NLS-Cre was injected into the PVN brain region of mouse,and the projection distribution of PVN axons in the PAG and MPA brain regions was observed after statistical analysis of its projection distribution through Image J.The optogenetic inhibitory virus mixture of AAV2/9-DIO-stGtACR2-EGFP and AAV2/9-mCrh-SV40 NLS-Cre targeting CRH neurons was injected into the PAG and MPA brain regions receiving the corresponding PVN projection,respectively,and the CRH neurons in the PAG and MPA brain regions were inhibited by optogenetic blue light 460 nm to observe the anxiety-related behaviors of mice under acute restraint stress.Results:Densely distributed CRHergic PVN axon terminals were observed in PAG and MPA brain regions.Optogenetic inhibition of CRH neurons in the PAG region of acute stress mice showed no significant change in social preference behavior.The eating latency decreased in the novelty-suppressed feeding test,and the escape latency increased under visual fear stimulation.Optogenetic inhibi-tion of CRH neurons in the MPA brain region showed no significant change in social preference,significantly decreased eating latency in the novelty-suppressed feeding test,and no significant change in escape latency under visual fear stim-ulation.Conclusion:CRH neurons in PAG and MPA brain regions downstream of PVN have differential regulation in a-cute stress-related anxiety behavior,but no difference in social behavior regulation,which provides theoretical support and basis for in-depth exploration of stress-related brain regions and cellular mechanisms.
