Analysis of differential expression profiles of circular RNA in oral squamous cell carcinoma and study on the carcinogenic mechanism of circ_PVT1
10.13591/j.cnki.kqyx.2025.09.003
- VernacularTitle:口腔鳞癌环状RNA差异表达谱分析及circ_PVT1致癌机制的研究
- Author:
Liwei WU
1
;
Yongle QIU
;
Jiahong ZHAO
;
Zhizheng ZHUANG
;
Kunshan LI
Author Information
1. 石家庄市第三医院口腔科,河北 石家庄(050000)
- Publication Type:Journal Article
- Keywords:
oral squamous cell carcinoma;
gene chip;
circ_PVT1;
Wnt3a/β-catenin;
pathway
- From:
STOMATOLOGY
2025;45(9):655-662
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the differential expression profile of circ_RNA in OSCC and to elucidate the molecular mechanism of circ_PVT1 on OSCC carcinogenesis.Methods The transcripts of 3 cases of OSCC and normal tissues were sequenced by high-throughput sequencing using circ_RNA expression profile chip,and the differential gene expression profiles were screened,and GO and KEGG enrichment analysis were performed.The expression level of PVT1 in OSCC tissues,human normal oral mucosal cells and OSCC cells was detected by qRT-PCR.The effect of PVT1 on the biological behavior of SCC-25 and SCC-9 cells was evaluated by MTT exper-iment,Transwell experiment and flow cytometry.The effect of PVT1 on the expression of key proteins in the Wnt3a/β-catenin pathway was evaluated by Western blot.The relationship between the expression of PVT1 and clinical pathological characteristics and prognosis of patients was further studied.Results A total of 403 differentially expressed circ_RNAs were screened by the chip,and the differen-tially expressed genes were enriched in pathways related to cancer progression.PVT1 was highly expressed in OSCC tissues and cells.Silencing PVT1 expression could inhibit the activation of the Wnt3a/β-catenin pathway,thereby effectively inhibiting the proliferation,migration,invasion and cell cycle of SCC-25 and SCC-9 cells and promoting apoptosis.PVT1 expression was only associated with lymph node metastasis and distant metastasis in patients,and those with high expression had a shorter PFS.Conclusion PVT1 promotes the progression of OSCC by regulating the activation of Wnt3a/β-catenin pathway.The research results provide new ideas for the diagnosis and treatment of OSCC.
