Laboratory study for an outbreak of foodborne diseases caused by Salmonella Java
10.3969/j.issn.1002-2694.2025.00.149
- VernacularTitle:Salmonella Java导致食源性疾病暴发事件的实验室检测
- Author:
Yuwei LIU
1
;
Wei DING
;
Shuang ZHANG
;
Yu ZHAO
;
Shoufei LI
;
Aixia YAN
;
Ying LI
;
Bo PANG
;
Xuerong SUN
;
Zhenpeng LI
Author Information
1. 北京市顺义区疾病预防控制中心,北京 101300;北京市顺义区疾病预防控制中心微生物感染性疾病检测工作站,北京 101300
- Publication Type:Journal Article
- Keywords:
Salmonella;
foodborne diseases;
fluorescence pcr;
virulence gene;
drug resistance gene
- From:
Chinese Journal of Zoonoses
2025;41(9):960-966
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to identify the Salmonella strains isolated from an outbreak of foodborne illness in a seafood buffet restaurant and analyze their pathogenic characteristics.Epidemiological data,fecal samples from patients and chefs,and food/environmental samples from the restaurant were analyzed.Research methods included bacterial culture,serotyping,quadruple fluo-rescence PCR identification,whole-genome sequencing and antibiotic susceptibility testing.The results showed that 4 S.Java strains(serotype 1,4,12∶b∶1,2;ST42)were isolated from two outbreak cases and two sporadic cases.All isolates exhibited similar genomic features,harboring 9 virulence islands and 98 virulence genes.Antimicrobial resistance profiling revealed streptomycin monoresis-tance,mediated by aac(6′)-Iy and aac(6′)-Iaa genes.In conclusion,this event was the first reported outbreak of foodborne illness caused by S.Java in China,indicating that S.Java may be prevalent in the surveyed district.The catering industry should optimize food handling and processing procedures and enhance the surveillance of high risk pathogens.Meanwhile,further studies should ad-dress differential diagnosis and pathogenic mechanism differences between S.Java and S.paratyphi B,which will facilitate evidence-based monitoring in China.
