Treatment of burns complicated with multidrug-resistant Pseudomonas aeruginosa infection by combining low-dose cocktail phage with antibiotics
10.3969/j.issn.1002-2694.2025.00.147
- VernacularTitle:小剂量鸡尾酒噬菌体联合抗菌药物治疗烧伤合并多耐药铜绿假单胞菌感染的研究
- Author:
Wujin CHEN
1
;
Sen ZHANG
;
Juanjuan DONG
;
Jingru ZHOU
;
Ping NI
;
Qin LI
;
Xuhua ZHANG
;
Lichuang CHEN
;
Wei YANG
;
Musong MO
;
Lidifu DILARE
Author Information
1. 新疆医科大学基础医学院形态中心,新疆医科大学医学科学研究所,乌鲁木齐 830017;新疆维吾尔自治区地方病分子生物学重点实验室,乌鲁木齐 830017
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
burn animal model;
phage therapy;
low-dose cocktail bacteriophage combined with anti-biotics
- From:
Chinese Journal of Zoonoses
2025;41(9):939-944
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the therapeutic effects of a low-dose phage cocktail combined with antibiotics on burn wounds in-fected with multidrug resistant Pseudomonas aeruginosa.Given the risk of sepsis caused by drug-resistant bacteria infection after burns and the limitations of antibiotic monotherapy,we constructed a mouse model of burns combined with Pseudomonas aeruginosa infection.A saline control group,phage cocktail monotherapy group,antibiotic monotherapy group,and combined treatment group were examined.The combined treatment group showed a synergistic effect on the 7th day after infection:this group of mice had a sig-nificantly lower pathogenic bacterial load in the skin and liver tissues than observed in the single drug treatment group,and showed the strongest bacterial clearance effect.Histopathological analysis indicated improved structural integrity of the skin tissue,as well as decreased infiltration of inflammatory cells,and no obvious tissue damage,in the combined treatment group.Detection of serum in-flammatory factors indicated that the levels of the pro-inflammatory factors IL-6 and TNF-α significantly decreased,whereas the level of anti-inflammatory factor IL-10 significantly increased.The combination of low-dose phage cocktail and antibiotics synergistically en-hanced antibacterial activity and ameliorated infection through a dual mechanism of direct removal of pathogens and regulation of the host immune response.Our findings provide an experimental basis for the optimal treatment of wounds infected with multidrug-resistant bacteria.
