Investigation and analysis of hepatitis E virus in rodents from selected regions of the Inner Mongolia Autonomous Region
10.3969/j.issn.1002-2694.2025.00.145
- VernacularTitle:内蒙古自治区部分地区鼠携带戊型肝炎病毒的调查分析
- Author:
Shanshan WANG
1
;
Xiaoyan SI
;
Mengmeng ZHANG
;
Man LUO
;
Zhen YAN
;
Lili LI
;
Xiaowei NAN
;
Zhongbing ZHANG
Author Information
1. 内蒙古自治区疾病预防控制中心(内蒙古自治区预防医学科学院),呼和浩特 010020;内蒙古自治区媒介生物控制学领先学科,呼和浩特 010020
- Publication Type:Journal Article
- Keywords:
mice;
hepatitis E virus;
nested PCR;
genetic evolutionary analysis;
Inner Mongolia Autonomous Region
- From:
Chinese Journal of Zoonoses
2025;41(9):925-931
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the prevalence of hepatitis E virus(HEV)in rodents within the Inner Mongolia Autonomous Region.In 2024,liver,spleen,kidney,and lung tissue samples were collected from rodents in 11 leagues and cities across the Inner Mongolia Autonomous Region,including Hohhot,Baotou,Hulunbuir,Xing'an League,Tongliao,Chifeng,Xilin Gol League,Ulan-qab,Ordos,Bayannur,and Wuhai.Nested PCR(RT-PCR)was used to detect the conserved regions of the HEV open reading frame 1(ORF1)gene.The RT-PCR-positive products were sequenced,and phylogenetic and homology analysis of the obtained sequences was performed.A total of 816 rodents were captured in this investigation,including 319 Rattus norvegicus(39.09%,319/816),206 Musmusculus(25.25%,206/816),and 140 Mongolian gerbils(17.16%,140/816).The HEV infection rate among rodents in the In-ner Mongolia region was 3.68%(30/816).Sequencing of the RT-PCR-positive results and analysis of the nucleotide sequences yielded 30 HEV-positive rodent samples.Phylogenetic analysis identified these sequences as belonging to the HEV-C1 genotype.The prevalence of HEV was observed in brown rats(Rattus norvegicus)in five leagues and cities within the Inner Mongolia region,includ-ing Xing'an League,Chifeng City,Hulunbuir City,Xilin Gol League,and Tongliao City,with infection rates of 16.67%,10.00%,5.98%,3.30%,and 2.50%,respectively.Brown rats,a species of house rats,frequently inhabit areas near human residences and have close interactions with humans and livestock.Studies have shown that multiple subtypes of HEV can cause zoonotic infections.Therefore,strengthening the monitoring of pathogens carried by rodents in residential environments and optimizing the prevention and control of rodent-borne diseases will be essential.Timely dissemination of relevant infectious disease knowledge to local communities will also be crucial,to decrease the risk of human infection.
