Establishment and assessment of deep vein thrombosis model in rats in a plateau hypoxic environment
10.3969/j.issn.1005-4847.2025.08.004
- VernacularTitle:高原缺氧环境大鼠深静脉血栓模型的建立与思考
- Author:
Xiaobo HAN
1
;
Yahao CHAI
;
Jiawei GAO
;
Xinkai DENG
;
Xiao LI
;
Jialin WU
;
Xiaoli HAN
;
Guoxiang LI
;
Yinjie ZHAO
;
Xi YANG
;
Qi AO
;
Lei ZHANG
;
Hongwei HAN
;
Zhixue LIU
Author Information
1. 新疆军区总医院急诊医学科,乌鲁木齐 830000
- Publication Type:Journal Article
- Keywords:
high altitude;
venous thrombosis;
animal model;
rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(8):1133-1143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat model of venous thrombosis in a plateau hypobaric hypoxic environment and to investigate the effect of this environment on venous thrombosis.Methods A total of 144 healthy male SD rats were assigned randomly to four groups(n=36 rats per group):a plains sham operation(A)group,plains operation(B)group,plateau altitude 6000 m+sham operation(C)group,and plateau altitude 6000 m+surgery(D)group.Rats in A and B groups were maintained in a plains normoxic environment,while rats in C and D groups C and D were subjected to a plateau environment.Rats in the surgical groups underwent quantitative constriction to incompletely obstruct the inferior vena cava blood flow.Each group was further divided into subgroups based on time:1,3,5,7,14,and 21 d(n=6 rats per group).Regular vascular ultrasound monitoring was conducted,and blood samples were taken for whole blood viscosity testing and the assessment of inflammatory indicators,including endothelin-1(ET-1),interleukin-6(IL-6)and tissue factor(TF).Coagulation function was evaluated through the activated partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen(FIB)and D-dimer.After the observation period,the experimental animals were sacrificed and the limbs were removed.Thrombus samples were stained with hematoxylin/eosin(HE),and the thrombus wet mass was measured.Results The thrombosis incidence was significantly higher in the plateau D group than in B group,accompanied by a marked increase in blood viscosity and hematocrit(P＜0.01).Additionally,levels of ET-1,IL-6,and TF were significantly elevated(P＜0.05),indicating a coagulation disorder.Conclusions A plateau hypoxic environment model can be successfully simulated by quantitative coarctation of the inferior vena cava,combined with a specialized environmental chamber.The findings of this study suggest that a plateau hypoxic environment promotes venous thrombosis.
