piRNA-823 inhibits high glucose induced endothelial-mesenchymal transition and an-giogenesis in human umbilical vein endothelial cells through TGF-β1/Smad2/3 signa-ling pathway
10.20039/j.cnki.1007-3949.2025.09.004
- VernacularTitle:piRNA-823通过TGF-β1/Smad2/3信号通路抑制高糖诱导的人脐静脉内皮细胞内皮-间充质转化和血管生成
- Author:
Yang HAN
1
;
Jianning QIN
1
;
Yao TAN
1
;
Letian YU
1
;
Shunlin QU
1
Author Information
1. 南华大学心血管疾病研究所动脉硬化学湖南省重点实验室湖南省动脉硬化性疾病国际科技创新合作基地,湖南省衡阳市 421001
- Publication Type:Journal Article
- Keywords:
piRNA-823;
endothelial-mesenchymal transition;
angiogenesis;
TGF-β1/Smad2/3 signaling pathway
- From:
Chinese Journal of Arteriosclerosis
2025;33(9):762-771
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the biological function and molecular mechanisms of piRNA-823 in the phenotypic transformation of human umbilical vein endothelial cells(HUVEC)induced by high glucose.Methods HUVEC were incubated in high glucose(33.3 mmol/L)culture medium for 72 h.The relative expression levels of piR-NA-823 were detected by RT-qPCR,the expression changes of endothelial cell markers,mesenchymal cell markers and proteins related to transforming growth factor-β1(TGF-β1)signaling pathway were detected by Western blot,the changes of cell migration ability were evaluated by scratch and Transwell assays,the formation of new angiogenesis were assessed through angiogenesis experiments.piRNA-823 mimic(overexpression of piRNA-823)were transfected into HUVEC to analyze their effects on high glucose induced endothelial-mesenchymal transition(EndMT)and angiogenesis.Further in-tervention was performed using TGF-β1 activator(SRI011381)and inhibitor(SB525334)to verify whether piRNA-823 ex-erts its effect by regulating the TGF-β1/Smad2/3 signaling pathway.Results piRNA-823 mimic significantly inhibited the viability,proliferation,migration and angiogenesis of HUVEC induced by high glucose.The piRNA-823 mimic inhibited high glucose induced EndMT in HUVEC,characterized by upregulation of endothelial cell markers and downregulation of mesenchymal cell markers.Scratch experiments,Transwell experiments and angiogenesis experiments further confirmed that piRNA-823 mimic could effectively reverse high glucose induced HUVEC proliferation,migration a-bility enhancement,and increase in the number of new angiogenesis.Mechanistic studies revealed that the TGF-β1 acti-vator partially reversed the protective effect of piRNA-823 mimic,whereas the TGF-β1 inhibitor enhanced its effect,sug-gesting that piRNA-823 exerts its regulatory role by suppressing the activation of the TGF-β1/Smad2/3 signaling pathway.Conclusion piRNA-823 significantly inhibits high glucose induced EndMT,proliferation,migration and angiogenesis in HUVEC by suppressing the activation of TGF-β1/Smad2/3 signaling pathway.
