Relationship between gut microbiota and onset of depression in chronic unpredictable mild stress model rats of both sexes
10.3969/j.issn.1005-4847.2025.08.005
- VernacularTitle:不同性别CUMS模型大鼠的肠道菌群差异与抑郁症发病关系
- Author:
Kanghong ZHU
1
;
Yumeng GAO
;
Mengxue HUANG
;
Liu YANG
;
Zizhan GAO
;
Hao CHU
;
Nan DENG
;
Ling HU
;
Zijian WU
Author Information
1. 安徽中医药大学针灸推拿学院,合肥 230012
- Publication Type:Journal Article
- Keywords:
depression;
chronic unpredictable mild stress;
gut microbiota;
sex difference
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(8):1144-1152
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the differences in gut microbiota in chronic unpredictable mild stress(CUMS)-induced depression model rats of both sexes,and to provide experimental evidence for exploring sex differences in depression onset.Methods Thirty-two healthy SD rats were divided randomly into four groups based on sex:Male control group(Control-M),Female control group(Control-F),Male model group(Model-M),and Female model group(Model-F)(n=8 rats per group).Rats in the control groups were fed without stimulation,while rats in the model groups were stimulated using the 28 d CUMS-induced depression method.After successful modeling,fresh feces were collected from all rats for high-throughput 16S rRNA sequencing.Behavioral observations were also conducted before and after preparing the model.Results The result of sucrose-preference,open-field,and forced-swimming tests differed significantly between the control and model groups.The result of the sucrose-preference test also differed between the sexes,while there was no difference in the open-field or forced-swimming test between the sexes.The α and β diversity of the gut microbiota genera showed an upward trend in the CUMS group compared with the control group.The ratio of Firmicutes/Bacteroidetes and the richness of the Roseburia and Lachnospiraceae_NK4A136_group were decreased in male rats but showed an increasing trend in female rats.Conclusions The ratio of Firmicutes/Bacteroidetes in the gut microbiota may be a key factor affecting the difference in the onset of depression between males and females,while the Roseburia and Lachnospiraceae_NK4A136_group be potential factors in correcting the gut microbiota and improving the symptoms of depression.
