Identification and analysisof drug resistance in Gordonia strains isolated from sputum samples in Henan Province
10.3969/j.issn.1002-2694.2025.00.111
- VernacularTitle:河南省痰标本戈登氏菌的分离鉴定与耐药分析
- Author:
Shaohua WANG
1
;
Wenjing CHANG
1
;
Ruyue SU
1
;
Xiaoguang MA
1
;
Danwei ZHENG
1
;
Yankun ZHU
1
;
Jie SHI
1
;
Dingyong SUN
1
;
Dongyang ZHAO
1
Author Information
1. 河南省疾病预防控制中心,郑州 450016
- Publication Type:Journal Article
- Keywords:
Actinomycetes;
Gordonia;
identification;
drug resistance
- From:
Chinese Journal of Zoonoses
2025;41(8):859-865
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at exploring the prevalence and drug sensitivity of Gordonia strains isolated from sputum samples in Henan Province,to provide data to aid in the prevention and treatment of Gordonia infection.A combination of 16S rDNA and sec A1 gene sequencing was used to identify the isolated strains,and susceptibility to16 drugs was determined with the broth microdilution method.A total of 21 strains were identified through 16S rDNA gene and sec A1 gene sequencing,including five strains of Gordonia broncians,eight strains of Gordonia paraphernivans,seven strains of Gordonia sputi,and one strain of Gordonia aichiensis.Drug sensi-tivity testing showed high Gordonia sensitivity to drugs such as ceftriaxone,linezolid,doxycycline,amoxicillin/clavulanic acid,mino-cycline,cefotaxime,trimethoprim/sulfamethoxazole,imipenem,tobramycin,and clarithromycin.The sensitivity rates of the isolated strains were 90.48%(19/21),100%(21/21),90.48%(19/21),90.48%(19/21),95.24%(20/21),90.48%(19/21),90.48%(19/21),90.48%(19/21),and 95.24%(20/21),respectively.Gordonia showed high resistance to rifampicin and cefepime,with rates of 28.57%(6/21)and 19.05%(4/21),respectively.Meanwhile,the resistance varied among bacterial strains.The resistance rate of G.sputi to rifampicin reached 71.43%(5/7),whereas that of G.parapffinivoras to cefepime was 37.5%(3/8).The main species of Gordo-nia isolated from sputum samples of patients in Henan Province were G.bronchialis,G.paraffinivoras,G.sputi,and G.aichiensis.Drug sensitivity tests indicated that drugs including amoxicillin/clavulanic acid,ceftriaxone,cefotaxime,tobramycin,clarithromycin,mi-nocycline,trimethoprim/sulfamethoxazole,linezolid,and doxycycline had good antibacterial effects against Gordonia.
