Influence of Leptospira interrogans infection on the expression of autophagy-related proteins Beclin-1,LC3B,and P62 in C3H/HeJ mice
10.3969/j.issn.1002-2694.2025.00.120
- VernacularTitle:问号钩端螺旋体感染对C3H/HeJ小鼠自噬相关蛋白Beclin-1、LC3B、P62表达的影响
- Author:
Cheng DU
1
;
Yan TAN
;
Ling XIE
;
Yuanyuan GU
;
Dong TANG
;
Xu CHEN
Author Information
1. 贵州中医药大学第二附属医院,贵阳 550003
- Publication Type:Journal Article
- Keywords:
Leptospira interrogans;
infection;
autophagy;
Beclin-1;
LC3B;
P62
- From:
Chinese Journal of Zoonoses
2025;41(8):832-837
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the influence of Leptospira interrogans infection on the expression of the autophagy-related proteinsmyosin-like BCL2 interacting protein(Beclin-1),microtubule-associated proteinlight chain 3B(LC3B),and sequestosome 1(P62).C3H/HeJ mice were infected with L.interrogansserovar stain Lai for construction of an animal model of leptospirosis.Silver strain-ing was used to detect leptospires in lung,liver,and kidney tissues of L.interrogans-infected mice.Histological changes in these tis-sues were detected with hematoxylin and eosin(HE)staining.The morphology of autophagosomes and leptospires in the lung,liver,and kidney tissues of L.interrogans-infected micewere determined through transmission electron microscopy.Expression of the autophagy-related proteins Beclin-1,LC3B,and P62 in the lung,liver,and kidney tissues of L.interrogans-infected C3H/HeJ mice was measured with immunohistochemistry.In these tissues,silver straining examination revealed leptospires.These tissues also showed histopathological changes typical of leptospirosis,such as pulmonary hemorrhage,extensive hepatocyte necrosis,both glomerular and tubular necrosis,and inflammatory cell infiltration;moreover,the leptospires were surrounded by autophagosomes.Immunohistochemi-cal examination indicated elevated expression of both Beclin-1 and LC3B in the lung,liver and kidney tissues of L.interrogans-infected mice(P<0.05),whereas the P62 level was significantly diminished in every tissue sample during L.interrogans infection(P<0.05).These results indicated that autophagy is activated during L.interrogans infection in the lung,liver,and kidney tissues of C3H/HeJ mice.
