Effects of"Gubenpeiyuan,Jianpi hewei"Acupuncture on JAK2/STAT5α/PGE2 Signaling Pathway Mediated by Leptin in Liver Tissue of Obese Rats
- VernacularTitle:固本培元、健脾和胃针刺法对肥胖大鼠肝组织Leptin介导的JAK2/STAT5α/PGE2信号通路的影响
- Author:
Juan BAO
1
;
Shaoyuan ZHUO
;
Caiyun LIAO
;
Sujie LIU
;
Hongzhen TANG
Author Information
- Publication Type:Journal Article
- Keywords: Acupuncture; Obesity; Leptin; JAK2/STAT5α/PGE2 signaling pathway; Molecular mechanism
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(9):2753-2760
- CountryChina
- Language:Chinese
- Abstract: To explore the molecular mechanisms underlying acupuncture-induced weight loss,the effects of acupuncture based on the principles of consolidating the foundation and strengthening the spleen and stomach on the Leptin-mediated JAK2/STAT5α/PGE2 signaling pathway in the liver tissue of obese rats were investigated.Methods Obese rat models were established and divided into six groups:Normal control group,normal control+AG490 group,obese model group,obese model+AG490 group,obese model+acupuncture group,and obese model+acupuncture+AG490 group,with 10 rats in each group.Serum Leptin and PGE2 levels were detected using ELISA,the expression of related proteins in liver tissue was assessed by Western blot,and changes in adipocyte morphology were observed through HE staining.Results In the obese model group,serum Leptin and PGE2 levels were significantly elevated,adipocyte area was enlarged,and the expression of JAK1,JAK2,STAT5α,COX1,and COX2 proteins in liver tissue significantly increased.Acupuncture treatment significantly reduced serum Leptin and PGE2 levels,decreased adipocyte area,and inhibited the expression of related proteins in liver tissue.Conclusion Acupuncture achieves weight loss by inhibiting the JAK2/STAT5α/PGE2 signaling pathway,improving Leptin resistance,reducing inflammatory responses,and decreasing fat accumulation.
