Analysis of pathogenic bacteria distribution and immune response of urinary tract infection after pyeloureteroplasty in children
10.3969/j.issn.1000-484X.2025.10.030
- VernacularTitle:儿童肾盂输尿管成形术后尿路感染病原菌分布及免疫反应变化分析
- Author:
Hailin WANG
1
;
Yi CHENG
1
;
Huixian ZHAO
1
;
Wenjie MA
1
;
Zhong HE
1
Author Information
1. 青海省妇女儿童医院普外科,西宁 810008
- Publication Type:Journal Article
- Keywords:
Pyeloureteroplasty;
Urinary tract infection;
Pathogenic bacteria;
Immunity
- From:
Chinese Journal of Immunology
2025;41(10):2488-2494
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of pathogenic bacteria distribution and immune response of urinary tract infection(UTI)after pyeloureteroplasty in children.Methods:A total of 150 children with obstructive hydronephroplasty who received pyeloureteroplasty in Qinghai Women and Children's Hospital from February 2020 to June 2023 were selected as the study objects.The postoperative urine of the children was collected,the pathogens were identified by automatic bacterial analyzer,and the patients were divided into infected group and uninfected group according to the infection status.The detection of pathogenic bacteria in children with UTI was analyzed.The clinical data of the two groups were compared,and the influencing factors of postoperative UTI were screened by Logistic regression analysis,and the prediction model was established and verified.The changes of immune response were compared between the two groups.Results:UTI occurred in 56 cases(37.33%)of 150 children who underwent pyeloureteroplasty.There were 58 strains of gram-negative bacteria,29 strains of gram-positive bacteria and 2 strains of fungi isolated.The main pathogenic bacteria was Escherichia coli.Multivariate Logistic regression analysis showed that serum creatinine(SCr)and hydronephrosis volume/renal volume(HV/RV)levels increased,urinary catheter retention time,urinary bag replacement time and hospital stay were risk factors for UTI after pyeloureteroplasty in children,increased age and elevated albumin(Alb)were protective factors(P<0.05).Through Bootstrap self-sampling,the prediction model had good differentiation and accuracy.There was no significant difference in the levels of CD4+,CD8+and IgM between the two groups before surgery(P>0.05),and the levels of CD4+,CD8+and IgM were signi-ficantly improved between the two groups after surgery,and the improvement degree of the uninfected group was significantly better than that of the infected group(P<0.05).Conclusion:The pathogens of UTI in children after pyeloureteroplasty are mainly gram-nega-tive bacteria,and the immune function is increased 7 days after the operation.
