Impacts of self-made Huanjie Mixture on airway remodeling and PI3K/AKT/mTOR signal pathway in asthma mice
10.3969/j.issn.1000-484X.2025.10.025
- VernacularTitle:自制缓解合剂对哮喘小鼠气道重塑及PI3K/AKT/mTOR信号通路的影响
- Author:
Qiang FU
1
;
Hong WANG
1
;
Shenqian ZHAO
1
Author Information
1. 山东青岛中西医结合医院药剂科,青岛 266002
- Publication Type:Journal Article
- Keywords:
Asthma;
Self-made Huanjie Mixture;
Airway remodeling;
PI3K/AKT/mTOR signal pathway
- From:
Chinese Journal of Immunology
2025;41(10):2456-2462
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impacts of self-made Huanjie Mixture on airway remodeling and PI3K/AKT/mTOR signal pathway in asthma mice.Methods:Sixty BALB/c mice were randomly grouped into control group,ovalbumin(OVA)group,self-made Huanjie Mixture low,medium and high dose groups(SHM-L,M,H groups)and PI3K inhibitor group(LY294002 group).In addition to the control group,other groups of mice were sensitized and challenged with OVA to build asthma models.After adminis-tration,the airway hyperresponsiveness of mice was detected by small animal lung function instrument;the levels of serum total IgE,IL-4 and IFN-γ in bronchoalveolar lavage fluid(BALF)were detected by ELISA;HE staining and PAS staining were applied to ob-serve the airway inflammation and mucus secretion in the lung tissue of mice;immunohistochemical staining was applied to detect the expression of α-SMA in mouse lung tissue;Western blot was applied to detect the expression of PI3K/AKT/mTOR signal pathway relat-ed proteins in mice lung tissue.Results:Compared with control group,the airway resistance(Rn),total IgE level,IL-4 level,inflam-mation score,the proportion of PAS positive staining area,the positive expression level of α-SMA protein,and the ratios of p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR in OVA group were increased,while IFN-γ level was decreased(P<0.05);compared with OVA group,Rn,total IgE level,IL-4 level,inflammation score,proportion of PAS positive staining area,positive expression level of α-SMA protein,and the ratios of p-PI3K/PI3K,p-AKT/AKT,p-mTOR/mTOR in SHM-L,M,H groups and LY294002 group were decreased,while IFN-γ level was increased(P<0.05),the changes of the above indexes in SHM-L,M and H groups showed a dose-dependent effect;compared with SHM-H group,the above indexes of LY294002 group mice had no obvious difference(P>0.05).Conclusion:The self-made Huanjie Mixture can reduce airway remodeling in asthma mice,and the inhibition of PI3K/AKT/mTOR signal pathway activation may be one of its mechanisms.
