Upregulated of PPIF associates with poor prognosis and immune infiltrates in lung adenocarcinoma
10.3969/j.issn.1000-484X.2025.10.022
- VernacularTitle:PPIF上调与肺腺癌预后不良和免疫浸润相关
- Author:
Ting DENG
1
;
Xiaoying LIU
1
Author Information
1. 大连医科大学附属第一医院全科医学,大连 116000
- Publication Type:Journal Article
- Keywords:
Lung adenocarcinoma;
PPIF;
Biomarker;
Immune infiltration
- From:
Chinese Journal of Immunology
2025;41(10):2435-2443,中插15
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression differences and clinical characteristics of peptide-prolyl cis-trans isomerase F(PPIF)gene in lung adenocarcinoma(LUAD),and to explore the correlation between PPIF and tumor prognosis and tumor microenvi-ronment infiltration to explore key biomarkers.Methods:TCGA,UALCAN databases and Kaplan-Meier survival analysis were used to study the expression difference,clinical characteristics,immune infiltration and prognostic value of PPIF gene in LUAD.The differ-ence of PPIF transcription and translation level in LUAD A549 cell line was verified by RT-qPCR and Western blot.At the same time,GO and KEGG were used for gene enrichment analysis,STRING database was used to construct protein interaction network,and Cyto-scape was used to visualize protein interaction network.Results:PPIF was proved to be highly expressed in LUAD,and methylation analysis showed that hypomethylation may lead to high expression of PPIF in LUAD.According to survival analysis,high PPIF expres-sion was associated with poor prognosis of LUAD.Pathway enrichment analysis showed that up-regulated PPIF played an important role in LUAD progression.Further analysis found that PPIF was associated with immune cell infiltration in LUAD,and PPIF overexpres-sion may affect the tumor microenvironment of LUAD patients.Conclusion:The high expression of PPIF in LUAD is related to the poor prognosis of LUAD and affects the immune infiltration level of LUAD,which may be a potential biomarker and clinical therapeu-tic target for LUAD patients.
