S-allylmercaptocysteine inhibits gastric cancer progression by regulating Th17/Treg immune balance
10.3969/j.issn.1000-484X.2025.10.020
- VernacularTitle:S-烯丙基巯基半胱氨酸通过调节Th17/Treg免疫平衡抑制胃癌进展
- Author:
Jian ZHAI
1
;
Jun LI
1
;
Fumin SHI
1
Author Information
1. 唐山市人民医院头颈肿瘤外科,唐山 063001
- Publication Type:Journal Article
- Keywords:
S-allylmercaptocysteine;
Th17/Treg;
Gastric cancer
- From:
Chinese Journal of Immunology
2025;41(10):2422-2426,2434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of S-allylmercaptocysteine(SAMC)on gastric cancer(GC)progression by regulating Th17/Treg immune balance.Methods:One hundred nude mice were randomly divided into control group(NC),low-dose SAMC group(SAMC-L),medium dose SAMC group(SAMC-M)and high-dose SAMC group(SAMC-H),with 25 mice in each group.Transplanted tumor model was established by subcutaneous injection of SGC-7901 cell suspension(0.2 ml,2×107 cells/ml)into the right shoulder of nude mice.Each group of nude mice was injected with corresponding doses of 0.9%sodium chloride solution and SAMC solution(100 mg/kg,200 mg/kg,400 mg/kg);injected once every 2 days and continuously administer for 3 weeks.After treatment,volume and mass of transplanted tumor were measured.Flow cytometry was used to detect proportion of Th17 and Treg cells in peripheral blood and transplanted tumors of nude mice;ELISA was used to detect IL-6,IL-10,IL-17,TGF-β1 levels in peripheral blood of nude mice;RT-qPCR was used to detect related markers mRNA levels of Th17 and Treg cells in peripheral blood and transplanted tumors of nude mice;Western blot was used to detect related markers protein levels of Th17 and Treg cells in transplanted tumors.Results:Compared with NC group,tumor volume and mass in SAMC-L group,SAMC-M group and SAMC-H group were decreased;Th17,Treg and Th17/Treg proportion in peripheral blood and transplanted tumors were decreased;IL-6,IL-10,IL-17 and TGF-β1 levels in peripheral blood were decreased;Foxp3,CTLA-4,GITR,RORγt,IL-17A mRNA and protein levels in peripheral blood and transplanted tumor were decreased,and there were significant differences in the above effects between different doses of SAMC groups(P<0.05).Conclusion:SAMC may inhibit GC progression by regulating Th17/Treg immune balance.
