Study of T11TS enhances immune function of rats infected with Cryptococcus neoformans by regulating CaN-NFAT in T lymphocytes
10.3969/j.issn.1000-484X.2025.10.019
- VernacularTitle:T11TS通过调节T淋巴细胞中CaN-NFAT增强新生隐球菌感染大鼠免疫功能的实验研究
- Author:
Lijuan WANG
1
;
Yanbin SU
1
;
Shanshan YE
1
;
Xiuwei XING
1
;
Jianshe CHEN
1
Author Information
1. 商丘医学高等专科学校基础医学教学部,商丘 476100
- Publication Type:Journal Article
- Keywords:
Cryptococcus neoformans;
Infection;
T lymphocyte;
Calcineurin;
Activated T lymphocyte nuclear factor
- From:
Chinese Journal of Immunology
2025;41(10):2416-2421
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze effects of T11TS in the treatment of Cryptococcus neoformans fungal infection and the regula-tion of T-cell calcineurin(CaN)-activated T lymphoid nuclear factor(NFAT)pathway.Methods:SD rats infected with Cryptococcus neoformans were treated with immunoenhancer T11TS.Flow cytometry,Western blot and nuclear translocation were used to detect the changes of T cell function and related signal pathway.Results:The number of Cryptococcus neoformans in lung and spleen of CT3 group was significantly less than Cryptococcus neoformans infection group,CT1 group and CT2 group(P<0.05).The results of flow cy-tometry showed that the expression levels of LCK,FYN,LAT,PLCγ1,CaN,NFAT and IL-2 in Cryptococcus neoformans infection group were significantly lower than control group(P<0.05).The expression levels of LCK,FYN,LAT,PLCγ1,CaN,NFAT and IL-2 of CT1,CT2 and CT3 groups were significantly higher than Cryptococcus neoformans infection group(P<0.05).The results of Western blot were similar to those of flow cytometry.Conclusion:T11TS in the treatment of Cryptococcus neoformans infection can enhance the immunity of the body by up-regulating the expression of related signal factors in the CaN-NFAT pathway of T lymphocytes,increasing the expression of NFAT and IL-2,stimulating the activation and proliferation of T lymphocytes.
