Mechanism research of IL-17 through P38/MK2-Cav1.2 signaling pathways involved in hypertension rats heart failure
10.3969/j.issn.1000-484X.2025.10.016
- VernacularTitle:IL-17通过P38/MK2-Cav1.2信号通路参与大鼠高血压心力衰竭机制研究
- Author:
Yonggang DING
1
;
Yihui LI
;
Tiannan JIN
;
Yingzi WANG
;
Tingting WU
Author Information
1. 兰州大学第二医院,兰州 730030
- Publication Type:Journal Article
- Keywords:
High blood pressure;
Heart failure;
IL-17;
P38/MK2-Cav1.2
- From:
Chinese Journal of Immunology
2025;41(10):2397-2402,2410
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and mechanism of IL-17 in hypertensive heart failure in rats.Methods:The spon-taneously hypertensive(SHR)model of hypertensive heart failure was established by abdominal aortic ligation.IL-17+IgG and IL-17 protein were injected intraperitoneally into the rats with hypertensive heart failure.After 4 weeks,cardiac structure and function were monitored,and peripheral blood and myocardial tissue were collected.The role and mechanism of IL-17 in hypertensive heart failure were studied by HE staining,immunohistochemistry,Western blot,qRT-PCR and ELISA.Results:After intraperitoneal injection of exogenous IL-17,the levels of IL-17,NT-proBNP,P38 and MK2 protein expressions were increased,and the levels of VEGF and Cav1.2 protein expression were decreased.Exogenous intraperitoneal injection of IL-17+IgG blocked IL-17,increased VEGF level and Cav1.2 protein expression,and decreased NT-proBNP level,P38 and MK2 protein expressions.Conclusion:IL-17 can be activated P38 lightning/MK2-Cav1.2 signaling pathways involved in high blood pressure,heart failure and cardiac function in rats damage;inhi-bition of IL-17 can effectively improve the cardiac function damage caused by hypertensive heart failure.
