Anti-inflammatory effects of celastrol on experimentally induced pulpitis in rats
10.3969/j.issn.1000-484X.2025.10.023
- VernacularTitle:雷公藤红素对大鼠实验性牙髓炎抗炎作用的研究
- Author:
Jiahao TANG
1
;
Lei PANG
1
;
Hao ZHANG
1
;
Li HE
1
Author Information
1. 十堰市人民医院(湖北医药学院附属人民医院),十堰 442000
- Publication Type:Journal Article
- Keywords:
Pulpitis;
Celastrol;
NF-κB p65;
TNF-α;
IL-1β
- From:
Chinese Journal of Immunology
2025;41(10):2444-2449
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate anti-inflammatory effects of celastrol as pulp capping agent on experimental pulpitis in rats and to explore its mechanism.Methods:Rat model of experimental pulpitis was established by lipopolysaccharide(LPS).A total of 28 rats were randomly divided into 4 groups(model group,calcium hydroxide group,celastrol group,drug mixture group).After 5 days,the rats were sacrificed and the maxillary and molar specimens were obtained.Pathological changes of pulp tissue were observed by HE staining,as well as the inflammation level was evaluated by a modified histological scoring system.Relative mRNA expression levels of TNF-α and IL-β in dental pulp were detected by qRT-PCR.Western blot was used to assess phosphorylation level of NF-κB p65 in dental pulp.Results:Histological scores of celastrol group and drug mixture group were significantly lower than those of model group and calcium hydroxide group(P<0.01),while there was no significant statistical differences between celastrol group and drug mixture group(P>0.05).Compared with model group,levels of TNF-α and IL-1β mRNA in pulp tissue of calcium hydroxide group,celastrol group and drug mixture group were decreased(P<0.05).Western blot showed that treatment of celastrol and mixed drugs could considerably reduce phosphorylation level of NF-κB p65 in rat pulp tissue.Conclusion:Celastrol can relieve experimental pulpitis in rats,and its mechanism may be related to the inhibition of NF-κB p65 activation and reduce the expressions of TNF-α and IL-1β.
