Improving effect of astragalus polysaccharide on recurrent spontaneous abortion rats by regulating PI3K/AKT signaling pathway
10.3969/j.issn.1000-484X.2025.10.008
- VernacularTitle:黄芪多糖调节PI3K/AKT信号通路对复发性流产大鼠的改善作用研究
- Author:
Xuemei CHEN
1
;
Yi YANG
1
;
Jingqiu GUO
1
;
Zhengyan ZHU
1
Author Information
1. 武汉市第三医院妇产科,武汉 430074
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharides;
Recurrent spontaneous abortion;
PI3K/AKT signaling pathway
- From:
Chinese Journal of Immunology
2025;41(10):2348-2353
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate improvement effect of astragalus polysaccharides(APS)on recurrent spontaneous abor-tion(RSA)in rats and its mechanism.Methods:Pregnant rats were randomly grouped into control group,RSA group,dexamethasone group,APS low,medium,high doses groups and LY294002 group.A RSA rat model was constructed,uterine organ index and embryo loss rate were calculated.HE staining was applied to observe histopathology changes of decidua.ELISA was applied to detect serum levels of estradiol(E2),progesterone(P),prolactin(PRL),cytokines such as IFN-γ,TNF-α,IL-4 and IL-10.Immunohisto-chemistry was applied to detect expression of decidua progesterone receptor(PR).Western blot was applied to detect expression of PI3K/AKT signaling pathway proteins of decidua.Results:Compared with control group,desquamated cells of rats in RSA group exhibit edema,disordered arrangement,nuclear pyknosis and disappearance,and decidua reduced interstitial blood vessels,uterine organ index,serum IL-4,IL-10,E2,P,PRL levels,decidua PR,p-PI3K,p-AKT expressions were obviously reduced,embryo loss rate,IFN-γ and TNF-α levels were obviously increased(P<0.05);compared with RSA group,morphology of decidual cells and interstitial blood vessels in dexamethasone group and APS low,medium and high doses groups were improved,uterine organ index,serum IL-4,IL-10,E2,P,PRL levels,decidua PR,p-PI3K,p-AKT expressions were obviously increased,embryo loss rate,serum IFN-γ and TNF-α levels were obviously reduced(P<0.05);LY294002 reversed improvement effect of high-dose APS on adverse pregnancy out-comes in RSA rats(P<0.05).Conclusion:APS can reduce miscarriage rate of RSA rats,regulate Th1/Th2 balance,whose mecha-nism may be related to activation of PI3K/AKT.
