Kisspeptin modulates Treg cell subsets at maternal-fetal interface in recurrent spontaneous abortion
10.3969/j.issn.1000-484X.2025.10.001
- VernacularTitle:Kisspeptin通过调节母胎界面Treg细胞亚群参与复发性流产的发生
- Author:
Yanhong YANG
1
;
Saizhe SONG
;
Sisi DING
;
Li YANG
;
Cuiping LIU
;
Hong ZHANG
Author Information
1. 苏州大学附属第二医院妇产科,苏州 215004;苏州大学附属第一医院江苏省临床免疫研究所,苏州 215021
- Publication Type:Journal Article
- Keywords:
Kisspeptin;
Recurrent spontaneous abortion;
Treg cells;
RNA-Seq
- From:
Chinese Journal of Immunology
2025;41(10):2305-2312
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether kisspeptin can influence the maternal-fetal interface regulatory T cells(Treg),thereby participating in the pathogenesis of recurrent spontaneous abortion(RSA).Methods:Normal pregnancy(NP)and RSA mice models were established,where NP mice received a tail vein injection of PBS(NP-PBS group),and RSA mice received a tail vein in-jection of PBS(RSA-PBS group)and active fragment of kisspeptin KP10(RSA-KP10 group),observing embryo absorption rates.Im-munohistochemistry was employed to assess expressions of kisspeptin and Foxp3 in mice uterine tissues.Peripheral blood Treg cells were isolated and expanded through magnetic bead separation.Intervention with KP10 and KP234(kisspeptin receptor antagonist)was administered,and flow cytometry was used to detect levels of IL-10 and TGF-β1 secretion by Treg cells,as well as differences in proliferation and apoptosis.RNA-Seq transcriptomic sequencing was conducted on uterine tissues from RSA-PBS group and RSA-KP10 group of mice.Differentially expressed genes(DEGs)were subjected to GO,KEGG and GSEA enrichment analyses.Results:Embryo absorption rate in RSA mice was higher than that in NP mice,the embryo absorption rate was decreased after tail vein injec-tion of KP10.Expressions of kisspeptin and Foxp3 in uterus of RSA mice was lower than that in NP mice,while increased after injec-tion of KP10.Kisspeptin could modulate the secretion of IL-10 and TGF-β1 by Treg cells,influencing their proliferation without affect-ing apoptosis.Enrichment analysis results showed that DEGs were mainly enriched in reproductive structure development,IL-17,and TGF-β signaling pathways.Conclusion:Kisspeptin can influence both the quantity and function of Treg cells,offering a new theoreti-cal foundation for investigating the pathogenesis and treatment of RSA.
