Comparative Analysis of Polyzoospermia and Normal Sperm Concentration In-fertility Semen Parameters and Assisted Reproductive Outcomes
10.3969/j.issn.1003-6946.2025.09.015
- VernacularTitle:多精子症与正常精子浓度不育精液参数和辅助生殖结局的对比分析
- Author:
Min LI
1
;
Ting LI
1
;
Xuan GAO
1
;
Sijie YANG
1
Author Information
1. 山东大学妇儿与生殖健康研究院 山东大学附属生殖医院医学检验科 山东大学生殖医学与子代健康全国重点实验室 山东大学国家辅助生殖与优生工程技术研究中心,山东济南 250012
- Publication Type:Journal Article
- Keywords:
Polyzoospermia;
Sperm nuclear protein maturity;
Semen analysis parameters;
Assisted reproduc-tive technology;
Pregnancy outcomes
- From:
Journal of Practical Obstetrics and Gynecology
2025;41(9):765-770
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the differences in semen parameters and the embryo outcomes in assisted reproductive technology between infertile patients with different levels of polyzoospermia and normal sperm con-centration,focusing on the relationship between polyzoospermia and sperm nucleoprotein maturity.Methods:A total of 212 couples with polyzoospermia(concentration ≥ 125 × 106/ml)who were admitted to the Insititute of Women,Children and Reproductive Health for infertility from October 12,2020 to August 25,2022 were selected.According to the concentration range,108 couples were divided into polyzoospermia group A[concentration(125-＜250)× 106ml]and 104 couples were divided into polyzoospermia group B(concentration ≥250 × 106/ml).Another 119 infertile couples with normal sperm concentration[(20-80)× 106/ml]were selected as the control group.The difference of semen parameters among the 3 groups male patient was compared,and the cor-relation between sperm concentration and the proportion of nucleoprotein-immature sperm was analyzed.The em-bryo development and pregnancy outcomes were compared between the 3 groups of women who received in vitro fertilization using intracytoplasmic sperm injection(ICSI).Results:①The age,days of abstinence,sperm concen-tration,total number of motile spermatozoa,total number of spermatozoa,total number of progressive spermato-zoa,and proportion of nuclear protein immature spermatozoa were all higher in group A and group B male patient of polyspermia compared to the control group,while the semen volume was lower compared to the control group(P＜0.01).The results of correlation analysis showed a positive correlation between sperm concentration and the proportion of nuclear protein immature spermatozoa(r=0.240,P=0.000).②The rate of diprokaryotic fertilization(2PN)was reduced after assisted reproduction in group A and group B with polyzoospermia,among which the rate of polyzoospermia in group B was lower than that in control group(P＜0.05),and the clinical pregnancy rate was lower than that in control group(51.2％vs.69.3％),and the biochemical pregnancy rate was higher than that in control group(9.3％vs.1.3％,P＜0.05).Conclusions:The total number of progressive spermatozoa and motile spermatozoa was higher in polyzoospermia patients than control group,but the nuclear protein imma-ture of spermatozoa was significantly higher than that in control group.When the sperm concentration exceeded 125 × 106/ml,the 2PN rate decreased,and when the sperm concentration exceeded 250 × 106/ml,the clinical pregnancy rate decreased significantly.Interventions should be taken before the implementation of ART to reduce the negative effects of high sperm concentration.
